Evidence map›Paper›PMID 25412310›Full record

ArticleCell death & disease2014

MicroRNA-194 reciprocally stimulates osteogenesis and inhibits adipogenesis via regulating COUP-TFII expression.

B-C Jeong, I-H Kang, Y-C Hwang, S-H Kim, J-T Koh

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell death & disease, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.

0numbers the graph read from it
0cells of the map it votes in
58citing papers in PubMed
4.3field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

58 citing papers in PubMed, 113 citations in OpenAlex.

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  12. Transit Amplifying Cells (TACs): a still not fully understood cell population.Frontiers in bioengineering and biotechnology · 2023
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

B-C Jeong1] Research Center for Biomineralization Disorders, and Dental Science Research Institute, School of Dentistry, Chonnam National University, Gwangju, Korea [2] Department of Pharmacology and Dental Therapeutics, School of Dentistry, Chonnam National University, Gwangju, Korea.
I-H Kang1] Research Center for Biomineralization Disorders, and Dental Science Research Institute, School of Dentistry, Chonnam National University, Gwangju, Korea [2] Department of Pharmacology and Dental Therapeutics, School of Dentistry, Chonnam National University, Gwangju, Korea.
Y-C Hwang1] Research Center for Biomineralization Disorders, and Dental Science Research Institute, School of Dentistry, Chonnam National University, Gwangju, Korea [2] Department of Conservative Dentistry, School of Dentistry, Chonnam National University, Gwangju, Korea.
S-H Kim1] Research Center for Biomineralization Disorders, and Dental Science Research Institute, School of Dentistry, Chonnam National University, Gwangju, Korea [2] Department of Oral Anatomy, School of Dentistry, Chonnam National University, Gwangju, Korea.
J-T Koh1] Research Center for Biomineralization Disorders, and Dental Science Research Institute, School of Dentistry, Chonnam National University, Gwangju, Korea [2] Department of Pharmacology and Dental Therapeutics, School of Dentistry, Chonnam National University, Gwangju, Korea.
Chonnam National University · KR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Osteoblasts and adipocytes are differentiated from common mesenchymal stem cells (MSCs) in processes which are tightly controlled by various growth factors, signaling molecules, transcriptional factors and microRNAs. Recently, chicken ovalbumin upstream promoter-transcription factor II (COUP-TFII) was identified as a critical regulator of MSC fate. In the present study, we aimed to identify some microRNAs (miR), which target COUP-TFII, and to determine the effects on MSCs fate. During osteoblastic or adipocytic differentiation from MSCs lineage cells, miR-194 expression was found to be reversal. In the cultures of mesenchymal C3H10T1/2 and primary bone marrow stromal cells, osteogenic stimuli increased miR-194 expression with accompanying decreases in COUP-TFII expression, whereas adipogenic stimuli reduced miR-194 expression with accompanying increases in COUP-TFII expression. A luciferase assay with COUP-TFII 3'-untranslated region (UTR) reporter plasmid, including the miR-194 binding sequences, showed that the introduction of miR-194 reduced the luciferase activity. However, it did not affect the activity of mutated COUP-TFII 3'-UTR reporter. Enforced expression of miR-194 significantly enhanced osteoblast differentiation, but inhibited adipocyte differentiation by decreasing COUP-TFII mRNA and protein levels. In contrast, inhibition of the endogenous miR-194 reduced matrix mineralization in the MSCs cultures, promoting the formation of lipid droplets by rescuing COUP-TFII expression. Furthermore, overexpression of COUP-TFII reversed the effects of miR-194 on the cell fates. Taken together, our results showed that miR-194 acts as a critical regulator of COUP-TFII, and can determinate the fate of MSCs to differentiate into osteoblasts and adipocytes. This suggests that miR-194 and COUP-TFII may be good target molecules for controlling bone and metabolic diseases.

Indexed as

3' Untranslated RegionsAdipocytesAdipogenesisAnimalsBone Marrow CellsBone Morphogenetic Protein 2Cell DifferentiationCell ProliferationCOUP Transcription Factor IICulture MediaFemurGene Expression RegulationGenes, ReporterHumansLuciferasesMesenchymal Stem Cells3' Untranslated RegionsBMP2 protein, humanBone Morphogenetic Protein 2COUP Transcription Factor IICulture MediaLuciferasesMicroRNAsMIRN194 microRNA, mouseNr2f2 protein, mouse

Identifiers

PMID25412310
PMCPMC4260743
OpenAlexW2015941704

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.