ArticleLab on a chip2015
A neutrophil treadmill to decouple spatial and temporal signals during chemotaxis.
Article in Lab on a chip, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed.
- Traveling-wave chemotaxis of neutrophil-like HL-60 cells.Molecular biology of the cell · 2025Article
- Self-extinguishing relay waves enable homeostatic control of human neutrophil swarming.Developmental cell · 2024Article
- Local negative feedback of Rac activity at the leading edge underlies a pilot pseudopod-like program for amoeboid cell guidance.PLoS biology · 2023Article
- T Cells Chemotaxis Migration Studies with a Multi-Channel Microfluidic Device.Micromachines · 2022Article
- Engineering physical microenvironments to study innate immune cell biophysics.APL bioengineering · 2022Review
- Signal integration in forward and reverse neutrophil migration: Fundamentals and emerging mechanisms.Current opinion in cell biology · 2021Review
- Microphysiological Systems for Studying Cellular Crosstalk During the Neutrophil Response to Infection.Frontiers in immunology · 2021Review
- Microfluidic devices for neutrophil chemotaxis studies.Journal of translational medicine · 2020Review
- Determining whether observed eukaryotic cell migration indicates chemotactic responsiveness or random chemokinetic motion.Journal of theoretical biology · 2017Article
- Temporal gradients limit the accumulation of neutrophils towards sources of chemoattractant.Microsystems & nanoengineering · 2017Article
- Big insights from small volumes: deciphering complex leukocyte behaviors using microfluidics.Journal of leukocyte biology · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
After more than 50 years of debates, the role of spatial and temporal gradients during cell chemotaxis is still a contentious matter. One major challenge is that when cells move in response to a heterogeneous chemical environment they are exposed to both spatial and temporal concentration changes. Even in the presence of perfectly stable chemical gradients, moving cells experience temporal changes of concentration simply by moving between locations with different chemical concentrations in a heterogeneous environment. Thus, the effects of the spatial and temporal stimuli cannot be dissociated and studied independently, hampering progress towards understanding the mechanisms of cell chemotaxis. Here we employ microfluidic and other engineering tools to build a system that accomplishes a function analogous to a treadmill at the cellular scale, holding a moving cell at a specified, unchanging location in a chemical gradient. Using this system, we decouple the spatial and temporal gradients around moving human neutrophils and find that temporal gradients are necessary for the directional persistence of human neutrophils during chemotaxis. Our results suggest that temporal chemoattractant changes are important during neutrophil migration and should be taken into account when deciphering the signalling pathways of cell chemotaxis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.