ArticleSensors (Basel, Switzerland)2014
Rapid immunoenzyme assay of aflatoxin B1 using magnetic nanoparticles.
Article in Sensors (Basel, Switzerland), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Revolutionizing Food Safety: A Systematic Review of Nanotechnology-Based Aflatoxin Detection (2010-2023).Nanotechnology, science and applications · 2026Review
- A core-satellite self-assembled SERS aptasensor used for ultrasensitive detection of AFBMikrochimica acta · 2025Article
- A comprehensive review of mycotoxins: Toxicology, detection, and effective mitigation approaches.Heliyon · 2024Review
- Comparison of efficacy and safety of different asparaginases in adult acute lymphoblastic leukemia based on nano-magnetic beads immunoassay.American journal of translational research · 2024Article
- Article
- Recent Progress on Techniques in the Detection of Aflatoxin BMolecules (Basel, Switzerland) · 2022Review
- Effect of processing on polyphenol profile, aflatoxin concentration and allergenicity of peanuts.Journal of food science and technology · 2021Article
- Advances in Antibody Preparation Techniques for Immunoassays of Total Aflatoxin in Food.Molecules (Basel, Switzerland) · 2020Review
- Recent Advances in Aflatoxins Detection Based on Nanomaterials.Nanomaterials (Basel, Switzerland) · 2020Review
- Article
- Portable Nanoparticle-Based Sensors for Food Safety Assessment.Sensors (Basel, Switzerland) · 2015Review
- Article
- Nominal effective immunoreaction volume of magnetic beads at single bead level.Journal of Zhejiang University. Science. BArticle
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The main limitations of microplate-based enzyme immunoassays are the prolonged incubations necessary to facilitate heterogeneous interactions, the complex matrix and poorly soluble antigens, and the significant sample dilutions often required because of the presence of organic extractants. This study presents the use of antibody immobilization on the surface of magnetic particles to overcome these limitations in the detection of the mycotoxin, aflatoxin B1. Features of the proposed system are a high degree of nanoparticle dispersion and methodologically simple immobilization of the antibodies by adsorption. Reactions between the immobilized antibodies with native and labeled antigens are conducted in solution, thereby reducing the interaction period to 5 min without impairing the analytical outcome. Adsorption of immunoglobulins on the surface of magnetic nanoparticles increases their stability in aqueous-organic media, thus minimizing the degree of sample dilution required. Testing barley and maize extracts demonstrated a limit of aflatoxin B1 detection equal to 20 pg/mL and total assay duration of 20 min. Using this method, only the 3-fold dilution of the initial methanol/water (60/40) extraction mixture in the microplate wells is necessary. The proposed pseudo-homogeneous approach could be applied toward immunodetection of a wide range of compounds.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.