ArticlePLoS genetics2014
An RNA-seq screen of the Drosophila antenna identifies a transporter necessary for ammonia detection.
Article in PLoS genetics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 92 papers.
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92 citing papers in PubMed, 146 citations in OpenAlex.
- Identification of markers facilitating the study of support cells in adult olfactory sensilla in Drosophila.Insect biochemistry and molecular biology · 2026Article
- Asymmetric Evolution of Antennal Cell Types Underlies a Derived Ammonia-Sensing Logic for Ecological Adaptation in Bactrocera dorsalis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Beat- and Side-family cell-surface molecules are expressed combinatorially in the partner neurons of the olfactory circuit in Drosophila.PLoS biology · 2026Article
- Comparative transcriptomic analysis of functional and metabolic changes in mass-reared and wildFrontiers in insect science · 2026Article
- Multilayer regulation underlies the functional precision and evolutionary potential of the olfactory system.Nature communications · 2025Article
- Glia-derived noncanonical fatty acid binding protein modulates brain lipid storage and clearance.Science advances · 2025Article
- New dimensions in the molecular genetics of insect chemoreception.Trends in genetics : TIG · 2025Review
- Cold-Sensing TRP Channels and Temperature Preference Modulate Ovarian Development in the Model OrganismInternational journal of molecular sciences · 2025Article
- An integrated anatomical, functional and evolutionary view of the Drosophila olfactory system.EMBO reports · 2025Article
- Volatile Organic Compounds in Biological Matrices as a Sensitive Weapon in Cancer Diagnosis.Pharmaceuticals (Basel, Switzerland) · 2025Review
- Cell-specific genetic expression profile of antennal glia in Drosophila reveals candidate genes in neuron-glia interactions.Scientific reports · 2025Article
- An integrated anatomical, functional and evolutionary view of thebioRxiv : the preprint server for biology · 2025Article
- Ectopic taste receptors in animal physiology: evolutionary conservation and functional diversification.Frontiers in cell and developmental biology · 2025Review
- Expression ofInsects · 2024Article
- Article
- Innexin expression and localization in the Drosophila antenna indicate gap junction or hemichannel involvement in antennal chemosensory sensilla.Cell and tissue research · 2024Article
- Molecular Characterization, Evolution and Expression Analysis of Ammonium Transporter from Four Closely RelatedLife (Basel, Switzerland) · 2024Article
- Article
- Identification of candidate genes associated with host-seeking behavior in the parasitoid wasp Diachasmimorpha longicaudata.BMC genomics · 2024Article
- Evolution of chemosensory tissues and cells across ecologically diverse Drosophilids.Nature communications · 2024Article
32 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Many insect vectors of disease detect their hosts through olfactory cues, and thus it is of great interest to understand better how odors are encoded. However, little is known about the molecular underpinnings that support the unique function of coeloconic sensilla, an ancient and conserved class of sensilla that detect amines and acids, including components of human odor that are cues for many insect vectors. Here, we generate antennal transcriptome databases both for wild type Drosophila and for a mutant that lacks coeloconic sensilla. We use these resources to identify genes whose expression is highly enriched in coeloconic sensilla, including many genes not previously implicated in olfaction. Among them, we identify an ammonium transporter gene that is essential for ammonia responses in a class of coeloconic olfactory receptor neurons (ORNs), but is not required for responses to other odorants. Surprisingly, the transporter is not expressed in ORNs, but rather in neighboring auxiliary cells. Thus, our data reveal an unexpected non-cell autonomous role for a component that is essential to the olfactory response to ammonia. The defective response observed in a Drosophila mutant of this gene is rescued by its Anopheles ortholog, and orthologs are found in virtually all insect species examined, suggesting that its role is conserved. Taken together, our results provide a quantitative analysis of gene expression in the primary olfactory organ of Drosophila, identify molecular components of an ancient class of olfactory sensilla, and reveal that auxiliary cells, and not simply ORNs, play an essential role in the coding of an odor that is a critical host cue for many insect vectors of human disease.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.