ArticleNature protocols2014
An integrated pipeline for the multidimensional analysis of branching morphogenesis.
Article in Nature protocols, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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Who cites it
27 citing papers in PubMed.
- Synthetic budding morphogenesis by optogenetic receptor tyrosine kinase signaling.bioRxiv : the preprint server for biology · 2026Article
- Counting glomeruli in human kidney specimens using ex vivo MRI without contrast agents.Magnetic resonance in medicine · 2025Article
- Maternal nutrition can rapidly rescue a nephron deficit in low birthweight offspring.npj metabolic health and disease · 2025Article
- Jamming of nephron-forming niches in the developing mouse kidney creates cyclical mechanical stresses.Nature materials · 2024Article
- Highly parallel production of designer organoids by mosaic patterning of progenitors.Cell systems · 2024Article
- Measurement of adhesion and traction of cells at high yield (MATCHY) reveals an energetic ratchet driving nephron condensation.bioRxiv : the preprint server for biology · 2024Article
- Nephron progenitors rhythmically alternate between renewal and differentiation phases that synchronize with kidney branching morphogenesis.bioRxiv : the preprint server for biology · 2024Article
- Rho/ROCK activity tunes cell compartment segregation and differentiation in nephron-forming niches.bioRxiv : the preprint server for biology · 2023Article
- The developing murine kidney actively negotiates geometric packing conflicts to avoid defects.Developmental cell · 2023Article
- Increasing mTORC1 Pathway Activity or Methionine Supplementation during Pregnancy Reverses the Negative Effect of Maternal Malnutrition on the Developing Kidney.Journal of the American Society of Nephrology : JASN · 2021Article
- Prenatal opioid exposure: The next neonatal neuroinflammatory disease.Brain, behavior, and immunity · 2020Article
- Article
- Morphogenesis of the kidney and lung requires branch-tip directed activity of the Adamts18 metalloprotease.Developmental biology · 2019Article
- Kidney organoids: accurate models or fortunate accidents.Genes & development · 2019Review
- Article
- Lin28 and let-7 regulate the timing of cessation of murine nephrogenesis.Nature communications · 2019Article
- DNA Methyltransferase 1 Controls Nephron Progenitor Cell Renewal and Differentiation.Journal of the American Society of Nephrology : JASN · 2019Article
- Branching morphogenesis in the developing kidney is not impacted by nephron formation or integration.eLife · 2018Article
- Hamartin regulates cessation of mouse nephrogenesis independently of Mtor.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Haploinsufficiency for the Six2 gene increases nephron progenitor proliferation promoting branching and nephron number.Kidney international · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Developmental branching morphogenesis establishes organ architecture, and it is driven by iterative interactions between epithelial and mesenchymal progenitor cell populations. We describe an approach for analyzing this interaction and how it contributes to organ development. After initial in vivo cell labeling with the nucleoside analog 5-ethynyl-2'-deoxyuridine (EdU) and tissue-specific antibodies, optical projection tomography (OPT) and confocal microscopy are used to image the developing organ. These imaging data then inform a second analysis phase that quantifies (using Imaris and Tree Surveyor software), models and integrates these events at a cell and tissue level in 3D space and across developmental time. The protocol establishes a benchmark for assessing the impact of genetic change or fetal environment on organogenesis that does not rely on ex vivo organ culture or section-based reconstruction. By using this approach, examination of two developmental stages for an organ such as the kidney can be undertaken by a postdoctoral-level researcher in 6 weeks, with a full developmental analysis in mouse achievable in 5 months.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.