Evidence map›Paper›PMID 25411727›Full record

ArticleInternational journal of environmental research and public health2014

Occurrence of virulence genes associated with diarrheagenic Escherichia coli isolated from raw cow's milk from two commercial dairy farms in the Eastern Cape Province, South Africa.

Lesley-Anne Caine, Uchechukwu U Nwodo, Anthony I Okoh, Roland N Ndip, Ezekiel Green

Abstract read
In one paragraph

Article in International journal of environmental research and public health, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Antimicrobial Resistance Phenotype ofBioMed research international · 2020
    Article
  9. Characterization of Non-O157Microorganisms · 2019
    Article
  10. Prevalence of diarrheagenicIranian journal of veterinary research · 2019
    Article
  11. Antimicrobial Resistance Profiles ofInternational journal of environmental research and public health · 2018
    Article
  12. Article
  13. Aerobic Mesophilic, Coliform,International journal of environmental research and public health · 2018
    Article
  14. Serogroups, subtypes and virulence factors of shiga toxin-producingGastroenterology and hepatology from bed to bench · 2018
    Article
  15. Article
  16. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Lesley-Anne CaineDepartments of Biochemistry and Microbiology, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa. 200906584@ufh.ac.za.
Uchechukwu U NwodoDepartments of Biochemistry and Microbiology, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa. UNwodo@ufh.ac.za.
Anthony I OkohDepartments of Biochemistry and Microbiology, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa. AOkoh@ufh.ac.za.
Roland N NdipDepartments of Biochemistry and Microbiology, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa. ndip3@yahoo.com.
Ezekiel GreenDepartments of Biochemistry and Microbiology, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa. EGreen@ufh.ac.za.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Escherichia coli remains a public health concern worldwide as an organism that causes diarrhea and its reservoir in raw milk may play an important role in the survival and transport of pathogenic strains. Diarrheagenic E. coli strains are diverse food-borne pathogens and causes diarrhea with varying virulence in humans. We investigated the prevalence of pathogenic E. coli in raw milk from two commercial dairy farms. Four hundred raw milk samples, 200 from each dairy farm, were screened for the presence of fliCH7, eagR, ial, eagg, lt, and papC genes. In dairy farm A, 100 E. coli were identified based on culture, oxidase and Gram staining, while 88 isolates from dairy farm B were identified in the same manner. Gene detection showed fliCH7 27 (54%) to be the highest gene detected from farm A and lt 2 (4%) to be the lowest. The highest gene detected in dairy farm B was fliCH7 16 (43.2%) and papC 1 (2.7%) was the least. The amplification of pathogenic genes associated with diarrheagenic E. coli from cows' raw milk demonstrates that potentially virulent E. coli strains are widely distributed in raw milk and may be a cause of concern for human health.

Indexed as

AnimalsDiarrheaEnteropathogenic Escherichia coliEscherichia coli InfectionsEscherichia coli ProteinsHumansMilkPrevalenceSouth AfricaVirulence FactorsEscherichia coli ProteinsVirulence Factors

Identifiers

PMID25411727
PMCPMC4245653

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.