ArticleChemistry Central journal2014
Characterization of the Vitrocell® 24/48 in vitro aerosol exposure system using mainstream cigarette smoke.
Article in Chemistry Central journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 71 citations in OpenAlex.
- Key challenges for in vitro testing of tobacco products for regulatory applications: Recommendations for dosimetry.Drug testing and analysis · 2023Article
- In Vitro Systems for Toxicity Evaluation of Microbial Volatile Organic Compounds on Humans: Current Status and Trends.Journal of fungi (Basel, Switzerland) · 2022Review
- Review
- Invited review: human air-liquid-interface organotypic airway tissue models derived from primary tracheobronchial epithelial cells-overview and perspectives.In vitro cellular & developmental biology. Animal · 2021Review
- A lower impact of an acute exposure to electronic cigarette aerosols than to cigarette smoke in human organotypic buccal and small airway cultures was demonstrated using systems toxicology assessment.Internal and emergency medicine · 2019Article
- A Device for measuring the in-situ response of Human Bronchial Epithelial Cells to airborne environmental agents.Scientific reports · 2019Article
- Comparative effects of a candidate modified-risk tobacco product Aerosol and cigarette smoke on human organotypic small airway cultures: a systems toxicology approach.Toxicology research · 2017Article
- Systems toxicology meta-analysis ofToxicology research · 2017Article
- In vitro exposure systems and dosimetry assessment tools for inhaled tobacco products: Workshop proceedings, conclusions and paths forward for in vitro model use.Alternatives to laboratory animals : ATLA · 2017Article
- Supporting evidence-based analysis for modified risk tobacco products through a toxicology data-sharing infrastructure.F1000Research · 2017Article
- Genotoxic properties of XLR-11, a widely consumed synthetic cannabinoid, and of the benzoyl indole RCS-4.Archives of toxicology · 2016Article
- Electronic cigarette aerosol induces significantly less cytotoxicity than tobacco smoke.Toxicology mechanisms and methods · 2016Article
- Assessment of in vitro COPD models for tobacco regulatory science: Workshop proceedings, conclusions and paths forward for in vitro model use.Alternatives to laboratory animals : ATLA · 2016Article
- Impact Assessment of Cigarette Smoke Exposure on Organotypic Bronchial Epithelial Tissue Cultures: A Comparison of Mono-Culture and Coculture Model Containing Fibroblasts.Toxicological sciences : an official journal of the Society of Toxicology · 2015Article
- A comparative assessment of cigarette smoke aerosols using an in vitro air-liquid interface cytotoxicity test.Inhalation toxicology · 2015Article
- Breathing in vitro: Designs and applications of engineered lung models.Journal of tissue engineeringReview
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundOnly a few exposure systems are presently available that enable cigarette smoke exposure of living cells at the air-liquid interface, of which one of the most versatile is the Vitrocell® system (Vitrocell® Systems GmbH). To assess its performance and optimize the exposure conditions, we characterized a Vitrocell® 24/48 system connected to a 30-port carousel smoking machine. The Vitrocell® 24/48 system allows for simultaneous exposure of 48 cell culture inserts using dilution airflow rates of 0-3.0 L/min and exposes six inserts per dilution. These flow rates represent cigarette smoke concentrations of 7-100%.
resultsBy characterizing the exposure inside the Vitrocell® 24/48, we verified that (I) the cigarette smoke aerosol distribution is uniform across all inserts, (II) the utility of Vitrocell® crystal quartz microbalances for determining the online deposition of particle mass on the inserts, and (III) the amount of particles deposited per surface area and the amounts of trapped carbonyls and nicotine were concentration dependent. At a fixed dilution airflow of 0.5 L/min, the results showed a coefficient of variation of 12.2% between inserts of the Vitrocell® 24/48 module, excluding variations caused by different runs. Although nicotine and carbonyl concentrations were linear over the tested dilution range, particle mass deposition increased nonlinearly. The observed effect on cell viability was well-correlated with increasing concentration of cigarette smoke.
conclusionsOverall, the obtained results highlight the suitability of the Vitrocell® 24/48 system to assess the effect of cigarette smoke on cells under air-liquid interface exposure conditions, which is closely related to the conditions occurring in human airways.
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