ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2014
In vivo identification of eugenol-responsive and muscone-responsive mouse odorant receptors.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 57 citations in OpenAlex.
- Article
- Distinct odorant receptor response patterns to aliphatic odorants in freely behaving mice.Chemical senses · 2025Article
- The influence of olfactory experience on the birthrates of olfactory sensory neurons with specific odorant receptor identities.Genesis (New York, N.Y. : 2000) · 2024Review
- Artificial Olfactory Biohybrid System: An Evolving Sense of Smell.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Review
- The eNOS isoform exhibits increased expression and activation in the main olfactory bulb of nNOS knock-out mice.Frontiers in cellular neuroscience · 2023Article
- Genetic variation in the human olfactory receptor OR5AN1 associates with the perception of musks.Chemical senses · 2023Article
- Dissociation of Mouse Olfactory Mucosae for Fluorescence-Activated Cell Sorting of Olfactory Sensory Neurons.Methods in molecular biology (Clifton, N.J.) · 2023Article
- A multivesicular body-like organelle mediates stimulus-regulated trafficking of olfactory ciliary transduction proteins.Nature communications · 2022Article
- Placing human gene families into their evolutionary context.Human genomics · 2022Review
- Functional analysis of human olfactory receptors with a high basal activity using LNCaP cell line.PloS one · 2022Article
- Odor coding in the mammalian olfactory epithelium.Cell and tissue research · 2021Review
- Article
- Encoding the Odor of Cigarette Smoke.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Article
- Mixture and concentration effects on odorant receptor response patterns in vivo.Chemical senses · 2020Article
- Modulation of the combinatorial code of odorant receptor response patterns in odorant mixtures.Molecular and cellular neurosciences · 2020Article
- Signal Detection and Coding in the Accessory Olfactory System.Chemical senses · 2018Review
- Signaling network between the dysregulated expression of microRNAs and mRNAs in propofol-induced developmental neurotoxicity in mice.Scientific reports · 2018Article
- Evolutionary ecology of chemosensation and its role in sensory drive.Current zoology · 2018Article
- Molecular mechanism of activation of human musk receptors OR5AN1 and OR1A1 by (Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Carbon chain shape selectivity by the mouse olfactory receptor OR-I7.Organic & biomolecular chemistry · 2018Article
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Authors and funding
7 authors at 5 institutions in 2 countries.
Funding
Abstract
Our understanding of mammalian olfactory coding has been impeded by the paucity of information about the odorant receptors (ORs) that respond to a given odorant ligand in awake, freely behaving animals. Identifying the ORs that respond in vivo to a given odorant ligand from among the ∼1100 ORs in mice is intrinsically challenging but critical for our understanding of olfactory coding at the periphery. Here, we report an in vivo assay that is based on a novel gene-targeted mouse strain, S100a5-tauGFP, in which a fluorescent reporter selectively marks olfactory sensory neurons that have been activated recently in vivo. Because each olfactory sensory neuron expresses a single OR gene, multiple ORs responding to a given odorant ligand can be identified simultaneously by capturing the population of activated olfactory sensory neurons and using expression profiling methods to screen the repertoire of mouse OR genes. We used this in vivo assay to re-identify known eugenol- and muscone-responsive mouse ORs. We identified additional ORs responsive to eugenol or muscone. Heterologous expression assays confirmed nine eugenol-responsive ORs (Olfr73, Olfr178, Olfr432, Olfr610, Olfr958, Olfr960, Olfr961, Olfr913, and Olfr1234) and four muscone-responsive ORs (Olfr74, Olfr235, Olfr816, and Olfr1440). We found that the human ortholog of Olfr235 and Olfr1440 responds to macrocyclic ketone and lactone musk odorants but not to polycyclic musk odorants or a macrocyclic diester musk odorant. This novel assay, called the Kentucky in vivo odorant ligand-receptor assay, should facilitate the in vivo identification of mouse ORs for a given odorant ligand of interest.
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