ArticleJournal of neurophysiology2015
Single-trial imaging of spikes and synaptic potentials in single neurons in brain slices with genetically encoded hybrid voltage sensor.
Article in Journal of neurophysiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 47 citations in OpenAlex.
- Distinct Patterns of Evoked Firing in Engram and Non-Engram Neurons in the Dentate Gyrus of Hippocampal Slices.Hippocampus · 2025Article
- Fxr1 Deletion from Cortical Parvalbumin Interneurons Modifies Their Excitatory Synaptic Responses.eNeuro · 2025Article
- Intrinsic and Synaptic Contributions to Repetitive Spiking in Dentate Granule Cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2024Article
- Inter and intralaminar excitation of parvalbumin interneurons in mouse barrel cortex.PloS one · 2024Article
- Velocity of conduction between columns and layers in barrel cortex reported by parvalbumin interneurons.Cerebral cortex (New York, N.Y. : 1991) · 2023Article
- Current Practice in Using Voltage Imaging to Record Fast Neuronal Activity: Successful Examples from Invertebrate to Mammalian Studies.Biosensors · 2023Review
- Unitary synaptic responses of parvalbumin interneurons evoked by excitatory neurons in the mouse barrel cortex.Cerebral cortex (New York, N.Y. : 1991) · 2023Article
- Imaging Voltage Globally and in Isofrequency Lamina in Slices of Mouse Ventral Cochlear Nucleus.eNeuro · 2023Article
- Chemical Targeting of Rhodol Voltage-Sensitive Dyes to Dopaminergic Neurons.ACS chemical neuroscience · 2022Article
- Linearly polarized excitation enhances signals from fluorescent voltage indicators.Biophysical journal · 2021Article
- A silicon-rhodamine chemical-genetic hybrid for far red voltage imaging from defined neurons in brain slice.RSC chemical biology · 2021Article
- Direct synaptic excitation between hilar mossy cells revealed with a targeted voltage sensor.Hippocampus · 2021Article
- The HaloTag as a general scaffold for far-red tunable chemigenetic indicators.Nature chemical biology · 2021Article
- Small Molecule-Protein Hybrid for Voltage Imaging via Quenching of Bioluminescence.ACS sensors · 2021Article
- A dark quencher genetically encodable voltage indicator (dqGEVI) exhibits high fidelity and speed.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Optical Studies of Action Potential Dynamics with hVOS probes.Current opinion in biomedical engineering · 2019Article
- Genetic voltage indicators.BMC biology · 2019Review
- Spying on Neuronal Membrane Potential with Genetically Targetable Voltage Indicators.Journal of the American Chemical Society · 2019Article
- Tethered Bichromophoric Fluorophore Quencher Voltage Sensitive Dyes.ACS sensors · 2018Article
- Mapping of excitatory and inhibitory postsynaptic potentials of neuronal populations in hippocampal slices using the GEVI, ArcLight.Journal of physics D: Applied physics · 2018Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Genetically encoded voltage sensors expand the optogenetics toolkit into the important realm of electrical recording, enabling researchers to study the dynamic activity of complex neural circuits in real time. However, these probes have thus far performed poorly when tested in intact neural circuits. Hybrid voltage sensors (hVOS) enable the imaging of voltage by harnessing the resonant energy transfer that occurs between a genetically encoded component, a membrane-tethered fluorescent protein that serves as a donor, and a small charged molecule, dipicrylamine, which serves as an acceptor. hVOS generates optical signals as a result of voltage-induced changes in donor-acceptor distance. We expressed the hVOS probe in mouse brain by in utero electroporation and in transgenic mice with a neuronal promoter. Under conditions favoring sparse labeling we could visualize single-labeled neurons. hVOS imaging reported electrically evoked fluorescence changes from individual neurons in slices from entorhinal cortex, somatosensory cortex, and hippocampus. These fluorescence signals tracked action potentials in individual neurons in a single trial with excellent temporal fidelity, producing changes that exceeded background noise by as much as 16-fold. Subthreshold synaptic potentials were detected in single trials in multiple distinct cells simultaneously. We followed signal propagation between different cells within one field of view and between dendrites and somata of the same cell. hVOS imaging thus provides a tool for high-resolution recording of electrical activity from genetically targeted cells in intact neuronal circuits.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.