ArticleProceedings of the National Academy of Sciences of the United States of America2014
Natural low-molecular mass organic compounds with oxidase activity as organocatalysts.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 25 citations in OpenAlex.
- Functional characterization of Gra-6 as a branch-point enzyme directing (R)-DNPA formation in BIQ polyketide biosynthesis.The Journal of antibiotics · 2026Article
- Methodology for awakening the potential secondary metabolic capacity in actinomycetes.Beilstein journal of organic chemistry · 2024Review
- Evidence of a role for CutRS and actinorhodin in the secretion stress response inMicrobiology (Reading, England) · 2023Article
- The Pleiotropic Regulator AdpA Regulates the Removal of Excessive Sulfane Sulfur inAntioxidants (Basel, Switzerland) · 2023Article
- Organocatalytic activity of granaticin and its involvement in bactericidal function.Scientific reports · 2022Article
- Phage tail-like nanostructures affect microbial interactions between Streptomyces and fungi.Scientific reports · 2021Article
- Hemoglobin catalyzes CoA degradation and thiol addition to flavonoids.Scientific reports · 2018Article
- Revisiting unexploited antibiotics in search of new antibacterial drug candidates: the case of γ-actinorhodin.Scientific reports · 2017Article
- Discovery of a sesamin-metabolizing microorganism and a new enzyme.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Streptomyces metabolites in divergent microbial interactions.Journal of industrial microbiology & biotechnology · 2016Review
- DNA Phosphorothioate Modification Plays a Role in Peroxides Resistance in Streptomyces lividans.Frontiers in microbiology · 2016Article
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Organocatalysts, low-molecular mass organic compounds composed of nonmetallic elements, are often used in organic synthesis, but there have been no reports of organocatalysts of biological origin that function in vivo. Here, we report that actinorhodin (ACT), a natural product derived from Streptomyces coelicolor A3(2), acts as a biocatalyst. We purified ACT and assayed its catalytic activity in the oxidation of L-ascorbic acid and L-cysteine as substrates by analytical methods for enzymes. Our findings were as follows: (i) oxidation reactions producing H2O2 proceeded upon addition of ACT to the reaction mixture; (ii) ACT was not consumed during the reactions; and (iii) a small amount (catalytic amount) of ACT consumed an excess amount of the substrates. Even at room temperature, atmospheric pressure, and neutral pH, ACT showed catalytic activity in aqueous solution, and ACT exhibited substrate specificity in the oxidation reactions. These findings reveal ACT to be an organocatalyst. ACT is known to show antibiotic activity, but its mechanism of action remains unknown. On the basis of our results, we propose that ACT kills bacteria by catalyzing the production of toxic levels of H2O2. We also screened various other natural products of bacterial, plant, and animal origins and found that several of the compounds exhibited catalytic activity, suggesting that living organisms produce and use these compounds as biocatalysts in nature.
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