ArticleThe Journal of biological chemistry2015
Trimeric structure of (+)-pinoresinol-forming dirigent protein at 1.95 Å resolution with three isolated active sites.
Article in The Journal of biological chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 1 synthesis or guideline pooled it, 67 citations in OpenAlex.
- Insights into the functional characterization of DIR proteins through genome-wide in silico and evolutionary studies: a systematic review.Functional & integrative genomics · 2023Pooled it
- miR398-Dirigent Module Negatively Regulates Cassava Resistance to Cassava Common Mosaic Virus by Affecting the Cell Wall Lignification and Thickness.Molecular plant pathology · 2025Article
- Evolutionary and functional analysis of the DIR gene family in Moso bamboo: insights into rapid shoot growth and stress responses.Frontiers in plant science · 2025Article
- Functional and evolutionary comparative analysis of the DIR gene family in Nicotiana tabacum L. and Solanum tuberosum L.BMC genomics · 2024Article
- Identification and functional characterization of the dirigent gene family in Phryma leptostachya and the contribution of PlDIR1 in lignan biosynthesis.BMC plant biology · 2023Article
- Genome-Wide Identification and Expression Pattern Analysis of Dirigent Members in the GenusInternational journal of molecular sciences · 2023Article
- Dirigent gene editing of gossypol enantiomers for toxicity-depleted cotton seeds.Nature plants · 2023Article
- Genome-wide identification and expression profiling analysis ofFrontiers in plant science · 2023Article
- The Crystal Structure of the Defense Conferring Rice ProteinBiomolecules · 2022Article
- Characterization of Peroxidase and Laccase Gene Families and In Silico Identification of Potential Genes Involved in Upstream Steps of Lignan Formation in Sesame.Life (Basel, Switzerland) · 2022Article
- Investigation of dirigent like domains from bacterial genomes.BMC bioinformatics · 2022Article
- Transgenic Forsythia plants expressing sesame cytochrome P450 produce beneficial lignans.Scientific reports · 2022Article
- Transcriptome analyses reveal the expression profile of genes related to lignan biosynthesis inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2022Article
- Biochemical and Initial Structural Characterization of the Monocot Chimeric Jacalin OsJAC1.International journal of molecular sciences · 2021Article
- Dirigent Proteins Guide Asymmetric Heterocoupling for the Synthesis of Complex Natural Product Analogues.Journal of the American Chemical Society · 2021Article
- Seed-coat protective neolignans are produced by the dirigent protein AtDP1 and the laccase AtLAC5 in Arabidopsis.The Plant cell · 2021Article
- Lignans of Sesame (Molecules (Basel, Switzerland) · 2021Review
- Molecular cloning and biochemical characterization of isoflav-3-ene synthase, a key enzyme of the biosyntheses of (+)-pisatin and coumestrol.Plant biotechnology (Tokyo, Japan) · 2020Article
- Pterocarpan synthase (PTS) structures suggest a common quinone methide-stabilizing function in dirigent proteins and proteins with dirigent-like domains.The Journal of biological chemistry · 2020Article
- The Resurgence of Dirigent Story: Time for a Bacterial Chapter.Current microbiology · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Control over phenoxy radical-radical coupling reactions in vivo in vascular plants was enigmatic until our discovery of dirigent proteins (DPs, from the Latin dirigere, to guide or align). The first three-dimensional structure of a DP ((+)-pinoresinol-forming DP, 1.95 Å resolution, rhombohedral space group H32)) is reported herein. It has a tightly packed trimeric structure with an eight-stranded β-barrel topology for each DP monomer. Each putative substrate binding and orientation coupling site is located on the trimer surface but too far apart for intermolecular coupling between sites. It is proposed that each site enables stereoselective coupling (using either two coniferyl alcohol radicals or a radical and a monolignol). Interestingly, there are six differentially conserved residues in DPs affording either the (+)- or (-)-antipodes in the vicinity of the putative binding site and region known to control stereoselectivity. DPs are involved in lignan biosynthesis, whereas dirigent domains/sites have been implicated in lignin deposition.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.