ArticleCellular microbiology2015
The co-transcriptome of uropathogenic Escherichia coli-infected mouse macrophages reveals new insights into host-pathogen interactions.
Article in Cellular microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 82 citations in OpenAlex.
- Reward-enhanced encoding improves relearning of forgotten associations.Scientific reports · 2018Trial
- Uropathogenic Escherichia coli proliferate as a coccoid morphotype inside human host cells.PLoS biology · 2025Article
- Exosomes derived from bladder epithelial cells infected with uropathogenic Escherichia coli increase the severity of urinary tract infections (UTIs) by impairing macrophage function.PLoS pathogens · 2024Article
- ProQ binding to small RNA RyfA promotes virulence and biofilm formation in avian pathogenic Escherichia coli.Veterinary research · 2023Article
- Are envelope stress responses essential for persistence to β-lactams inAntimicrobial agents and chemotherapy · 2023Article
- "Omics" Technologies - What Have They Told Us About UropathogenicFrontiers in cellular and infection microbiology · 2022Review
- Article
- Cross-species RNA-seq for deciphering host-microbe interactions.Nature reviews. Genetics · 2021Review
- The RyfA small RNA regulates oxidative and osmotic stress responses and virulence in uropathogenic Escherichia coli.PLoS pathogens · 2021Article
- A Comparative Transcriptomic Analysis of Human Placental Trophoblasts in Response to Pathogenic and ProbioticThe Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2021Article
- Copper primes adaptation of uropathogenic Escherichia coli to superoxide stress by activating superoxide dismutases.PLoS pathogens · 2020Article
- Spatially distinct physiology of Bacteroides fragilis within the proximal colon of gnotobiotic mice.Nature microbiology · 2020Article
- Bacterial genetics and molecular pathogenesis in the age of high throughput DNA sequencing.Current opinion in microbiology · 2020Review
- Article
- Article
- Bacterial Diversity of Diabetic Foot Ulcers: Current Status and Future Prospectives.Journal of clinical medicine · 2019Review
- Differential interleukin-1β induction by uropathogenic Escherichia coli correlates with its phylotype and serum C-reactive protein levels in Korean infants.Scientific reports · 2019Article
- DNA methylation analysis of porcine mammary epithelial cells reveals differentially methylated loci associated with immune response against Escherichia coli challenge.BMC genomics · 2019Article
- Replication of Crohn's Disease MucosalPathogens (Basel, Switzerland) · 2019Article
- Acetate metabolic requirement of avian pathogenic Escherichia coli promotes its intracellular proliferation within macrophage.Veterinary research · 2019Article
Corrections and comments
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Authors and funding
12 authors at 6 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Urinary tract infections (UTI) are among the most common infections in humans. Uropathogenic Escherichia coli (UPEC) can invade and replicate within bladder epithelial cells, and some UPEC strains can also survive within macrophages. To understand the UPEC transcriptional programme associated with intramacrophage survival, we performed host-pathogen co-transcriptome analyses using RNA sequencing. Mouse bone marrow-derived macrophages (BMMs) were challenged over a 24 h time course with two UPEC reference strains that possess contrasting intramacrophage phenotypes: UTI89, which survives in BMMs, and 83972, which is killed by BMMs. Neither of these strains caused significant BMM cell death at the low multiplicity of infection that was used in this study. We developed an effective computational framework that simultaneously separated, annotated and quantified the mammalian and bacterial transcriptomes. Bone marrow-derived macrophages responded to the two UPEC strains with a broadly similar gene expression programme. In contrast, the transcriptional responses of the UPEC strains diverged markedly from each other. We identified UTI89 genes up-regulated at 24 h post-infection, and hypothesized that some may contribute to intramacrophage survival. Indeed, we showed that deletion of one such gene (pspA) significantly reduced UTI89 survival within BMMs. Our study provides a technological framework for simultaneously capturing global changes at the transcriptional level in co-cultures, and has generated new insights into the mechanisms that UPEC use to persist within the intramacrophage environment.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.