ArticlePlant cell reports2015
Suppression of Arabidopsis AtPUB30 resulted in increased tolerance to salt stress during germination.
Article in Plant cell reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 35 citations in OpenAlex.
- Genome-wide analysis of CHYR gene family and BnA03.CHYR.1 functional verification under salt stress in Brassica napus L.BMC plant biology · 2025Article
- Arabidopsis ubiquitin ligase PUB41 positively regulates ABA-mediated seed dormancy and drought response.Physiology and molecular biology of plants : an international journal of functional plant biology · 2024Article
- Genome-Wide Identification and Expression Analysis of thePlants (Basel, Switzerland) · 2024Article
- Genome wide association study reveals new genes for resistance to striped stem borer in rice (Frontiers in plant science · 2024Article
- Genome-wide identification ofFrontiers in genetics · 2024Article
- Classification and Expression Profile of the U-Box E3 Ubiquitin Ligase Enzyme Gene Family in Maize (Plants (Basel, Switzerland) · 2022Article
- Review
- How Many Faces Does the Plant U-Box E3 Ligase Have?International journal of molecular sciences · 2022Review
- Genome-Wide Identification and Expression Analysis of the Plant U-Box Protein Gene Family inFrontiers in genetics · 2021Article
- Classification of barley U-box E3 ligases and their expression patterns in response to drought and pathogen stresses.BMC genomics · 2019Article
- Article
- Identification of wheat stress-responding genes and TaPR-1-1 function by screening a cDNA yeast library prepared following abiotic stress.Scientific reports · 2019Article
- AtARRE, an E3 ubiquitin ligase, negatively regulates ABA signaling in Arabidopsis thaliana.Plant cell reports · 2018Article
- Identification of SNPs and Candidate Genes Associated With Salt Tolerance at the Seedling Stage in Cotton (Frontiers in plant science · 2018Article
- A major QTL on chromosome 7HS controls the response of barley seedling to salt stress in the Nure × Tremois population.BMC genetics · 2017Article
- AtAIRP2 E3 Ligase Affects ABA and High-Salinity Responses by Stimulating Its ATP1/SDIRIP1 Substrate Turnover.Plant physiology · 2017Article
- The U-box family genes in Medicago truncatula: Key elements in response to salt, cold, and drought stresses.PloS one · 2017Article
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Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
key messageThe Arabidopsis U-box E3 Ub ligase AtPUB30 participates in the salt stress tolerance as a negative factor in an ABA-independent manner during germination. Based on the in silico expression data, the U-box protein 30 (AtPUB30) from Arabidopsis thaliana was identified as a gene that responds to salt stress. The deduced AtPUB30 protein consists of 448 amino acids with a single U-box motif and five ARM-repeat domains. An in vitro self-ubiquitination assay demonstrated that bacterially expressed AtPUB30 exhibited E3 ubiquitin (Ub) ligase activity and that the U-box domain was essential for the activity. Real-time qRT-PCR and promoter-GUS analyses showed that AtPUB30 was induced by high salinity, but not by drought, cold, or abscisic acid (ABA), in roots but not in shoots. These results suggest that AtPUB30 is an Arabidopsis U-box E3 Ub ligase, the expression of which is selectively enhanced by salt stress in roots. T-DNA-inserted loss-of-function atpub30 mutant plants (atpub30-1 and atpub30-2) were more tolerant to salt stress in the germination stage, as identified by radicle emergence, cotyledon opening, and more vigorous early root growth relative to wild-type plants. Thus, it is likely that AtPUB30 plays a negative role in high salinity tolerance in the germination process. Wild type and mutant plants displayed very similar germination rates when treated with ABA, suggesting that the action of AtPUB30 in the germination stage is ABA independent. The post-germination growth of NaCl-stressed wild type and mutant plants were indistinguishable. Overall, our data suggest that the Arabidopsis U-box E3 Ub ligase AtPUB30 participates in the salt stress tolerance as a negative factor in the germination stage in root tissues.
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