ArticlePlant cell reports2015
Characterization of a small constitutive promoter from Arabidopsis translationally controlled tumor protein (AtTCTP) gene for plant transformation.
Article in Plant cell reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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27 citing papers in PubMed, 58 citations in OpenAlex.
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- Rational design of induced regeneration via somatic embryogenesis in the absence of exogenous phytohormones.The Plant cell · 2025Article
- Identification and application of bioparts for plant synthetic biology.Molecules and cells · 2025Review
- WGCNA analysis reveals hub genes in the Hemarthria compressa roots in response to waterlogging stress.Scientific reports · 2025Article
- CRISPRi-based circuits to control gene expression in plants.Nature biotechnology · 2025Article
- Optimizing Promoters and Subcellular Localization for Constitutive Transgene Expression in Marchantia polymorpha.Plant & cell physiology · 2024Article
- Functional Characterization ofPlants (Basel, Switzerland) · 2024Article
- A non-invasive method to predict drought survival in Arabidopsis using quantum yield under light conditions.Plant methods · 2023Article
- Systematic Comparison of Plant Promoters inInternational journal of molecular sciences · 2022Article
- Synthetic memory circuits for stable cell reprogramming in plants.Nature biotechnology · 2022Article
- Multiplex genome editing inPlant direct · 2021Article
- Development of efficient synthetic promoters derived from pararetrovirus suitable for translational research.Planta · 2021Article
- Seed-specific activity of the Arabidopsis β-glucosidase 19 promoter in transgenic Arabidopsis and tobacco.Plant cell reports · 2021Article
- Article
- Identification of transcription factors interacting with a 1274 bp promoter of MaPIP1;1 which confers high-level gene expression and drought stress Inducibility in transgenic Arabidopsis thaliana.BMC plant biology · 2020Article
- Article
- An update and perspectives on the use of promoters in plant genetic engineering.Journal of biosciences · 2020Review
- Identification of a 119-bp Promoter of the Maize Sulfite Oxidase Gene (International journal of molecular sciences · 2019Article
- Isolation and identification of a vegetative organ-specific promoter from maize.Physiology and molecular biology of plants : an international journal of functional plant biology · 2019Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
key messageA plant-derived 0.3 kb constitutive promoter was obtained from AtTCTP expression analysis, and successfully applied to the expression of a selectable marker gene for production of transgenic creeping bentgrass plants. The isolation and use of an efficient promoter is essential to develop a vector system for efficient genetic transformation of plants, and constitutive promoters are particularly useful for the expression of selectable marker genes. In this study, we characterized a small size of the constitutive promoter from the expression analysis of Arabidopsis thaliana translationally controlled tumor protein (AtTCTP) gene. Histochemical and fluorometric GUS analyses revealed that a 303 bp upstream region from the start codon of the AtTCTP gene showed strong GUS expression throughout all plant tissues, which is approximately 55 % GUS activity compared with the cauliflower mosaic virus 35S promoter (35Spro). To examine the possible application of this promoter for the development of genetically engineered crops, we introduced pCAMBIA3301 vector harboring the 0.3 kb promoter of AtTCTP (0.3kbpro) that was fused to the herbicide resistance BAR gene (0.3kb pro ::BAR) into creeping bentgrass. Our transformation results demonstrate that transgenic creeping bentgrass plants with herbicide resistance were successfully produced using 0.3kb pro ::BAR as a selectable marker. Northern blot analysis revealed that the transgenic plants with 0.3kb pro ::BAR showed reduced but comparable expression levels of BAR to those with 35S pro ::BAR. Moreover, the transcription activity of the 0.3 kb promoter could be increased by the fusion of an enhancer sequence. These results indicate that the 0.3 kb AtTCTP promoter can be used as a plant-derived constitutive promoter for the expression of selectable marker genes, which facilitates its use as an alternative to the 35S promoter for developing genetically engineered crops.
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