ArticleDaru : journal of Faculty of Pharmacy, Tehran University of Medical Sciences2014
Osteogenic potential of punica granatum through matrix mineralization, cell cycle progression and runx2 gene expression in primary rat osteoblasts.
Article in Daru : journal of Faculty of Pharmacy, Tehran University of Medical Sciences, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 49 citations in OpenAlex.
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- Ultrasound-Mediated Biotransfection of Engineered Bone Marrow Mesenchymal Stem Cells in Treated Bone Defects through Intracellular Cavitation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Early osteogenic differentiation of human dental stem cells by gelatin/calcium phosphate- Punica granatum nanocomposite scaffold.BMC biotechnology · 2025Article
- Bioactivity and biomedical applications of pomegranate peel extract: a comprehensive review.Frontiers in pharmacology · 2025Review
- Materials Suitable for Osteochondral Regeneration.ACS omega · 2024Review
- In Vitro and In Vivo Bone-Forming Effect of a Low-Molecular-Weight Collagen Peptide.Journal of microbiology and biotechnology · 2024Article
- The Association of Nanostructured Carbonated Hydroxyapatite with Denatured Albumin and Platelet-Rich Fibrin: Impacts on Growth Factors Release and Osteoblast Behavior.Journal of functional biomaterials · 2024Article
- Moringa oleifera leaf extract induces osteogenic-like differentiation of human osteosarcoma SaOS2 cells.Journal of traditional and complementary medicine · 2022Article
- Anticancer potential of Phoenix dactylifera L. seed extract in human cancer cells and pro-apoptotic effects mediated through caspase-3 dependent pathway in human breast cancer MDA-MB-231 cells: an in vitro and in silico investigation.BMC complementary medicine and therapies · 2022Article
- Histological Evaluation of the Effect of Local Application ofInternational journal of biomaterials · 2022Article
- In Vitro Biological Evaluation of a Fabricated Polycaprolactone/Pomegranate Electrospun Scaffold for Bone Regeneration.ACS omega · 2021Article
- Integrated Omics Reveals the Orchestrating Role of Calycosin in Danggui Buxue Tang, a Herbal Formula Containing Angelicae Sinensis Radix and Astragali Radix, in Inducing Osteoblastic Differentiation and Proliferation.Frontiers in pharmacology · 2021Article
- Genistein contributes to cell cycle progression and regulates oxidative stress in primary culture of osteoblasts along with osteoclasts attenuation.BMC complementary medicine and therapies · 2020Article
- Induction of apoptosis by piperine in human cervical adenocarcinoma via ROS mediated mitochondrial pathway and caspase-3 activation.EXCLI journal · 2019Article
- Incorporation of silica nanoparticles to PLGA electrospun fibers for osteogenic differentiation of human osteoblast-like cells.Regenerative biomaterials · 2018Article
- Dihydrotestosterone, a robust promoter of osteoblastic proliferation and differentiation: understanding of time-mannered and dose-dependent control of bone forming cells.Iranian journal of basic medical sciences · 2017Article
- Development of a new rutin nanoemulsion and its application on prostate carcinoma PC3 cell line.EXCLI journal · 2017Article
- Influence of the intensity and loading time of direct current electric field on the directional migration of rat bone marrow mesenchymal stem cells.Frontiers of medicine · 2016Article
- Chemically synthesized CdSe quantum dots inhibit growth of human lung carcinoma cells via ROS generation.EXCLI journal · 2016Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundOsteoporosis is one of the prevalent diseases in ageing populations. Due to side effects of many chemotherapeutic agents, there is always a need to search for herbal products to treat the disorder. Punica granatum (PG) represent a potent fruit-bearing medicinal herb which exerted valuable anti-osteoporotic activities. The present study was carried out to validate the in vitro osteogenic effects of the PG seed extract in primary calvarial osteoblast cultures harvested from neonatal rats.
methodsThe ethanolic extract of PG was subjected to evaluate cell proliferation, regeneration, mineralization and formation of collagen matrix using MTT, alkaline phosphatase, Alizarin Red-S staining and Sirius Red dye, respectively. Cell cycle progression and osteogenic gene Runx2 expression were carried out by flow cytometry and real time PCR, respectively.
resultsExposure of different concentrations (10-100 μg/ml) of the extract on osteoblastic cells showed characteristic morphological changes and increment in cell number. A significant growth in cell proliferation, ALP activity, collagen contents and matrix mineralization of osteoblasts in a dose dependent manner (p < 0.05), suggested that PG has a stimulatory effect on osteoblastic bone formation or potential activity against osteoporosis. In addition, PG extract also enhanced DNA content in S phase of cell cycle and Runx2 gene expression level in osteoblasts.
conclusionThe data clearly indicated that PG promoting bone cell proliferation and differentiation in primary osteoblasts might be due to elevating the osteogenic gene Runx2 expression. The present study provides an evidence for PG could be a promising herbal medicinal candidate that able to develop drugs for osteoporosis.
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