ArticlePloS one2014
Frequency and fitness consequences of bacteriophage φ6 host range mutations.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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Who cites it
22 citing papers in PubMed, 41 citations in OpenAlex.
- Phage host range: determinants, dynamics and applications.Nature reviews. Microbiology · 2026Review
- Narrowed host ranges do not constrain future host range expansion in RNA phage Φ6.Journal of virology · 2026Article
- Oxidative stress constrains evolution of bacteriophage host-range diversity.The ISME journal · 2026Article
- Marine Bacteriophages as Next-Generation Therapeutics: Insights into Antimicrobial Potential and Application.Viruses · 2025Review
- Article
- Experimental Evolution Studies in Φ6 Cystovirus.Viruses · 2024Review
- Risk of infection due to airborne virus in classroom environments lacking mechanical ventilation.PloS one · 2024Article
- A meta-analysis highlights the idiosyncratic nature of tradeoffs in laboratory models of virus evolution.Virus evolution · 2024Article
- Review
- Isolation, Partial Characterization and Application of Bacteriophages in Eradicating Biofilm Formation byPathogens (Basel, Switzerland) · 2022Article
- Humidity Reduces Rapid and Distant Airborne Dispersal of Viable Viral Particles in Classroom Settings.Environmental science & technology letters · 2022Article
- Systematic analysis of putative phage-phage interactions on minimum-sized phage cocktails.Scientific reports · 2022Article
- Interactions between bacterial and phage communities in natural environments.Nature reviews. Microbiology · 2022Review
- Effects of historical co-infection on host shift abilities of exploitative and competitive viruses.Evolution; international journal of organic evolution · 2021Article
- A virophage cross-species infection through mutant selection represses giant virus propagation, promoting host cell survival.Communications biology · 2020Article
- Existing Host Range Mutations Constrain Further Emergence of RNA Viruses.Journal of virology · 2019Article
- Recessive Host Range Mutants and Unsusceptible Cells That Inactivate Virions without Genome Penetration: Ecological and Technical Implications.Journal of virology · 2019Article
- The Evolution of Molecular Compatibility between Bacteriophage ΦX174 and its Host.Scientific reports · 2018Article
- Genetic hurdles limit the arms race between Prochlorococcus and the T7-like podoviruses infecting them.The ISME journal · 2017Article
- Evolutionary ecology of virus emergence.Annals of the New York Academy of Sciences · 2017Review
Corrections and comments
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Authors and funding
13 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Viruses readily mutate and gain the ability to infect novel hosts, but few data are available regarding the number of possible host range-expanding mutations allowing infection of any given novel host, and the fitness consequences of these mutations on original and novel hosts. To gain insight into the process of host range expansion, we isolated and sequenced 69 independent mutants of the dsRNA bacteriophage Φ6 able to infect the novel host, Pseudomonas pseudoalcaligenes. In total, we found at least 17 unique suites of mutations among these 69 mutants. We assayed fitness for 13 of 17 mutant genotypes on P. pseudoalcaligenes and the standard laboratory host, P. phaseolicola. Mutants exhibited significantly lower fitnesses on P. pseudoalcaligenes compared to P. phaseolicola. Furthermore, 12 of the 13 assayed mutants showed reduced fitness on P. phaseolicola compared to wildtype Φ6, confirming the prevalence of antagonistic pleiotropy during host range expansion. Further experiments revealed that the mechanistic basis of these fitness differences was likely variation in host attachment ability. In addition, using computational protein modeling, we show that host-range expanding mutations occurred in hotspots on the surface of the phage's host attachment protein opposite a putative hydrophobic anchoring domain.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.