ArticlePloS one2014
Sperm preservation by freeze-drying for the conservation of wild animals.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The trial behind it
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Who cites it
14 citing papers in PubMed.
- Optimizing South African giraffe Epididymal sperm preservation techniques".Veterinary and animal science · 2026Article
- Biobanking biodiversity: recent strategies, challenges, and opportunities.The Journal of reproduction and development · 2026Review
- Freeze-Drying and Room Temperature Preservation of Mammalian Sperm.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Method for long-term room temperature storage of mouse freeze-dried sperm.Scientific reports · 2025Article
- The Sixth Mass Extinction and Amphibian Species Sustainability Through Reproduction and Advanced Biotechnologies, Biobanking of Germplasm and Somatic Cells, and Conservation Breeding Programs (RBCs).Animals : an open access journal from MDPI · 2024Review
- 40 'wild' years: the current reality and future potential of assisted reproductive technologies in wildlife species.Animal reproduction · 2024Review
- A novel, simplified method to prepare and preserve freeze-dried mouse sperm in plastic microtubes.The Journal of reproduction and development · 2023Article
- Correlation between Post-Thaw Spermatozoa Quality of the Endangered Javan Banteng with OPN Gene Expression.Veterinary medicine international · 2023Article
- Resurrecting biodiversity: advanced assisted reproductive technologies and biobanking.Reproduction & fertility · 2022Review
- Cryopreservation of Giraffe Epidydimal Spermatozoa Using Different Extenders and Cryoprotectants.Animals : an open access journal from MDPI · 2022Article
- Ultrastructural analysis of Lyophilized Human Spermatozoa.JBRA assisted reproduction · 2021Article
- Reproductive technologies for the generation and maintenance of valuable animal strains.The Journal of reproduction and development · 2018Review
- Dry Preservation of Spermatozoa: Considerations for Different Species.Biopreservation and biobanking · 2017Review
- Simple gamete preservation and artificial reproduction of mammals using micro-insemination techniques.Reproductive medicine and biology · 2015Review
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sperm preservation is a useful technique for the maintenance of biological resources in experimental and domestic animals, and in wild animals. A new preservation method has been developed that enables sperm to be stored for a long time in a refrigerator at 4 °C. Sperm are freeze-dried in a solution containing 10 mM Tris and 1 mM EDTA. Using this method, liquid nitrogen is not required for the storage and transportation of sperm. We demonstrate that chimpanzee, giraffe, jaguar, weasel and the long-haired rat sperm remain viable after freeze-drying. In all species, pronuclei were formed after the injection of freeze-dried sperm into the mouse oocytes. Although preliminary, these results may be useful for the future establishment of "freeze-drying zoo" to conserve wild animals.
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Registered trials
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