ArticleCell regeneration (London, England)2014
Production of transgenic pigs over-expressing the antiviral gene Mx1.
Article in Cell regeneration (London, England), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 26 citations in OpenAlex.
- A new strategy to reduce human influenza infection: creating pigs with partial resistance to influenza A virus infection by targetingJournal of biomedical research · 2025Article
- Pigs lackingFrontiers in genome editing · 2023Article
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- Anti-Classical Swine Fever Virus Strategies.Microorganisms · 2021Review
- Current and prospective control strategies of influenza A virus in swine.Porcine health management · 2021Review
- Extensive germline genome engineering in pigs.Nature biomedical engineering · 2021Article
- Generation of Marker-FreeGenes · 2020Article
- Antiviral activity of ISG15 against classical swine fever virus replication in porcine alveolar macrophages via inhibition of autophagy by ISGylating BECN1.Veterinary research · 2020Article
- A Wide-Ranging Antiviral Response in Wild Boar Cells Is Triggered by Non-coding Synthetic RNAs From the Foot-and-Mouth Disease Virus Genome.Frontiers in veterinary science · 2020Article
- Transgenic pigs expressing β-xylanase in the parotid gland improve nutrient utilization.Transgenic research · 2019Article
- Harnessing genomic information for livestock improvement.Nature reviews. Genetics · 2019Review
- Birth and death of Mx genes and the presence/absence of genes regulating Mx transcription are correlated with the diversity of anti-pathogenicity in vertebrate species.Molecular genetics and genomics : MGG · 2019Article
- Classical swine fever virus replicated poorly in cells from MxA transgenic pigs.BMC veterinary research · 2016Article
- Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The myxovirus resistance gene (Mx1) has a broad spectrum of antiviral activities. It is therefore an interesting candidate gene to improve disease resistance in farm animals. In this study, we report the use of somatic cell nuclear transfer (SCNT) to produce transgenic pigs over-expressing the Mx1 gene. These transgenic pigs express approximately 15-25 times more Mx1 mRNA than non-transgenic pigs, and the protein level of Mx1 was also markedly enhanced. We challenged fibroblast cells isolated from the ear skin of transgenic and control pigs with influenza A virus and classical swine fever virus (CFSV). Indirect immunofluorescence assay (IFA) revealed a profound decrease of influenza A proliferation in Mx1 transgenic cells. Growth kinetics showed an approximately 10-fold reduction of viral copies in the transgenic cells compared to non-transgenic controls. Additionally, we found that the Mx1 transgenic cells were more resistant to CSFV infection in comparison to non-transgenic cells. These results demonstrate that the Mx1 transgene can protect against viral infection in cells of transgenic pigs and indicate that the Mx1 transgene can be harnessed to develop disease-resistant pigs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.