ArticleeLife2014
Transcription mediated insulation and interference direct gene cluster expression switches.
Article in eLife, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 40 citations in OpenAlex.
- Nucleotide resolution 4-thiouridine sequencing by SNU-Seq and sf4sU-Seq reveals the transcriptional responsiveness of an epigenetically primed human genome.Nucleic acids research · 2026Article
- The hidden power of antisense long non-coding RNAs: a dive into a novel regulatory layer mediated by double-stranded RNA formation.RNA biology · 2025Review
- Functional analysis and transcriptional profiling of non-coding RNAs in yeast.The Biochemical journal · 2025Review
- Optogenetic control of horizontally acquired genes prevent stuck fermentations in yeast.Microbiology spectrum · 2025Article
- Size fractionated NET-Seq reveals a conserved architecture of transcription units around yeast genes.Yeast (Chichester, England) · 2024Article
- Article
- Promoter-pervasive transcription causes RNA polymerase II pausing to boost DOG1 expression in response to salt.The EMBO journal · 2023Article
- Unique features of transcription termination and initiation at closely spaced tandem human genes.Molecular systems biology · 2022Article
- Transcriptional neighborhoods regulate transcript isoform lengths and expression levels.Science (New York, N.Y.) · 2022Article
- Functional and transcriptional profiling of non-coding RNAs in yeast reveal context-dependent phenotypes and in trans effects on the protein regulatory network.PLoS genetics · 2021Article
- Efficient termination of nuclear lncRNA transcription promotes mitochondrial genome maintenance.eLife · 2018Article
- Antisense transcription-dependent chromatin signature modulates sense transcript dynamics.Molecular systems biology · 2018Article
- Native elongating transcript sequencing reveals global anti-correlation between sense and antisense nascent transcription in fission yeast.RNA (New York, N.Y.) · 2018Article
- Article
- Intragenic Enhancers Attenuate Host Gene Expression.Molecular cell · 2017Article
- Review
- Paf1 Has Distinct Roles in Transcription Elongation and Differential Transcript Fate.Molecular cell · 2017Article
- Transcription-coupled changes to chromatin underpin gene silencing by transcriptional interference.Nucleic acids research · 2016Article
- The molecular basis of metabolic cycles and their relationship to circadian rhythms.Nature structural & molecular biology · 2016Review
- Selective suppression of antisense transcription by Set2-mediated H3K36 methylation.Nature communications · 2016Article
Corrections and comments
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Authors and funding
14 authors at 2 institutions in 2 countries.
Funding
Abstract
In yeast, many tandemly arranged genes show peak expression in different phases of the metabolic cycle (YMC) or in different carbon sources, indicative of regulation by a bi-modal switch, but it is not clear how these switches are controlled. Using native elongating transcript analysis (NET-seq), we show that transcription itself is a component of bi-modal switches, facilitating reciprocal expression in gene clusters. HMS2, encoding a growth-regulated transcription factor, switches between sense- or antisense-dominant states that also coordinate up- and down-regulation of transcription at neighbouring genes. Engineering HMS2 reveals alternative mono-, di- or tri-cistronic and antisense transcription units (TUs), using different promoter and terminator combinations, that underlie state-switching. Promoters or terminators are excluded from functional TUs by read-through transcriptional interference, while antisense TUs insulate downstream genes from interference. We propose that the balance of transcriptional insulation and interference at gene clusters facilitates gene expression switches during intracellular and extracellular environmental change.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.