ArticleJournal of visualized experiments : JoVE2014
Assessment of selective mRNA translation in mammalian cells by polysome profiling.
Article in Journal of visualized experiments : JoVE, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
33 citing papers in PubMed.
- FracFixR: a compositional statistical framework for absolute proportion estimation between fractions in RNA sequencing data.Bioinformatics (Oxford, England) · 2026Article
- Article
- Polysome profiling is an extensible tool for the analysis of bulk protein synthesis, ribosome biogenesis, and the specific steps in translation.Molecular biology of the cell · 2025Article
- Leishmania Ribosomal Protein (RP) paralogous genes compensate each other's expression maintaining protein native levels.PloS one · 2024Article
- Effective extraction of polyribosomes exposes gene expression strategies in primary astrocytes.Nucleic acids research · 2023Article
- Interferon regulates neural stem cell function at all ages by orchestrating mTOR and cell cycle.EMBO molecular medicine · 2023Article
- Cytoplasmic FMR1 interacting protein (CYFIP) family members and their function in neural development and disorders.Molecular biology reports · 2021Review
- mRNP granule proteins Fmrp and Dcp1a differentially regulate mRNP complexes to contribute to control of muscle stem cell quiescence and activation.Skeletal muscle · 2021Article
- Polysome Profiling without Gradient Makers or Fractionation Systems.Journal of visualized experiments : JoVE · 2021Article
- Curcumin prevents obesity by targeting TRAF4-induced ubiquitylation in mEMBO reports · 2021Article
- Feedback Regulation ofInternational journal of molecular sciences · 2021Article
- Artificial miRNAs targeting CAG repeat expansion in ORFs cause rapid deadenylation and translation inhibition of mutant transcripts.Cellular and molecular life sciences : CMLS · 2021Article
- Depletion of eukaryotic initiation factor 5B (eIF5B) reprograms the cellular transcriptome and leads to activation of endoplasmic reticulum (ER) stress and c-Jun N-terminal kinase (JNK).Cell stress & chaperones · 2021Article
- SINEUP long non-coding RNA acts via PTBP1 and HNRNPK to promote translational initiation assemblies.Nucleic acids research · 2020Article
- Modulation of Shoot Phosphate Level and Growth byPlant physiology · 2020Article
- YTHDF2 promotes the liver cancer stem cell phenotype and cancer metastasis by regulating OCT4 expression via m6A RNA methylation.Oncogene · 2020Article
- Polysome-associated lncRNAs during cardiomyogenesis of hESCs.Molecular and cellular biochemistry · 2020Article
- Article
- QKI regulates adipose tissue metabolism by acting as a brake on thermogenesis and promoting obesity.EMBO reports · 2020Article
- IDH1 fine-tunes cap-dependent translation initiation.Journal of molecular cell biology · 2019Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Regulation of protein synthesis represents a key control point in cellular response to stress. In particular, discreet RNA regulatory elements were shown to allow to selective translation of specific mRNAs, which typically encode for proteins required for a particular stress response. Identification of these mRNAs, as well as the characterization of regulatory mechanisms responsible for selective translation has been at the forefront of molecular biology for some time. Polysome profiling is a cornerstone method in these studies. The goal of polysome profiling is to capture mRNA translation by immobilizing actively translating ribosomes on different transcripts and separate the resulting polyribosomes by ultracentrifugation on a sucrose gradient, thus allowing for a distinction between highly translated transcripts and poorly translated ones. These can then be further characterized by traditional biochemical and molecular biology methods. Importantly, combining polysome profiling with high throughput genomic approaches allows for a large scale analysis of translational regulation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.