Evidence map›Paper›PMID 25407345›Full record

ArticleJournal of visualized experiments : JoVE2014

Production and targeting of monovalent quantum dots.

Daeha Seo, Justin Farlow, Kade Southard, Young-Wook Jun, Zev J Gartner

Open access · bronzeAbstract readVideo-Audio Media
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of visualized experiments : JoVE, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.1field-weighted citation impact, top 46% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 2 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Daeha SeoDepartment of Otolaryngology, University of California, San Francisco; Department of Chemistry, University of California, Berkeley; Materials Science Division, Lawrence Berkeley National Laboratory.
Justin FarlowDepartment of Pharmaceutical Chemistry, University of California, San Francisco; Tetrad Graduate Program, University of California, San Francisco; Center for Systems and Synthetic Biology, University of California, San Francisco.
Kade SouthardDepartment of Otolaryngology, University of California, San Francisco; Department of Pharmaceutical Chemistry, University of California, San Francisco; Chemistry and Chemical Biology Graduate Program, University of California, San Francisco.
Young-Wook JunDepartment of Otolaryngology, University of California, San Francisco; Chemistry and Chemical Biology Graduate Program, University of California, San Francisco; YJun@ohns.ucsf.edu.
Zev J GartnerDepartment of Pharmaceutical Chemistry, University of California, San Francisco; Tetrad Graduate Program, University of California, San Francisco; Center for Systems and Synthetic Biology, University of California, San Francisco; Chemistry and Chemical Biology Graduate Program, University of California, San Francisco; zevgartner@gmail.com.
University of California, San Francisco · US

Funding

Rewiring Therapeutic Cells: User-Controlled T Cells and Stem CellsP50GM081879 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI MARSHALL, WALLACE · 2010 to 2017
$19.0M
RESEARCH TRAINING IN CHEMISTRY AND CHEMICAL BIOLOGYT32GM064337 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI CRAIK, CHARLES SCOTT · 2002 to 2021
$7.8M
Total synthesis of the human mammary glandDP2HD080351 · NICHD · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI GARTNER, ZEV JORDAN · 2013 to 2013
$2.4M
Single Molecule Imaging of Guided Axonal Development using Plasmon NanorulersR21EB015088 · NIBIB · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI JUN, YOUNG-WOOK · 2012 to 2013
$414k
Small, modular, and monovalent Quantum Dots for single molecule imaging of NotchR21EB018044 · NIBIB · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI GARTNER, ZEV JORDAN, JUN, YOUNG-WOOK · 2014 to 2015
$411k
NIBIB NIH HHS 1R21EB018044NIBIB NIH HHS 5R21EB015088-02NIBIB NIH HHS R21 EB015088NIBIB NIH HHS R21 EB018044NICHD NIH HHS DP2 HD080351NIGMS NIH HHS P50 GM081879NIGMS NIH HHS T32 GM064337
6 · The paper itself

Abstract

The multivalent nature of commercial quantum dots (QDs) and the difficulties associated with producing monovalent dots have limited their applications in biology, where clustering and the spatial organization of biomolecules is often the object of study. We describe here a protocol to produce monovalent quantum dots (mQDs) that can be accomplished in most biological research laboratories via a simple mixing of CdSe/ZnS core/shell QDs with phosphorothioate DNA (ptDNA) of defined length. After a single ptDNA strand has wrapped the QD, additional strands are excluded from the surface. Production of mQDs in this manner can be accomplished at small and large scale, with commercial reagents, and in minimal steps. These mQDs can be specifically directed to biological targets by hybridization to a complementary single stranded targeting DNA. We demonstrate the use of these mQDs as imaging probes by labeling SNAP-tagged Notch receptors on live mammalian cells, targeted by mQDs bearing a benzylguanine moiety.

Indexed as

Cell Line, TumorDNAHumansPhosphorothioate OligonucleotidesQuantum DotsDNAPhosphorothioate Oligonucleotides

Identifiers

PMID25407345
PMCPMC4309134
OpenAlexW2061584347

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.