ArticleJournal of visualized experiments : JoVE2014
Fabricating complex culture substrates using robotic microcontact printing (R-µCP) and sequential nucleophilic substitution.
Article in Journal of visualized experiments : JoVE, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Advancing models of neural development with biomaterials.Nature reviews. Neuroscience · 2021Review
- Article
- Methods of Micropatterning and Manipulation of Cells for Biomedical Applications.Micromachines · 2017Review
- Recent Advances in the Analysis of Single Cells.Analytical chemistry · 2017Review
- Micropatterned, clickable culture substrates enable in situ spatiotemporal control of human PSC-derived neural tissue morphology.Chemical communications (Cambridge, England) · 2015Article
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Authors and funding
4 authors.
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No grant is acknowledged in the PubMed record.
Abstract
In tissue engineering, it is desirable to exhibit spatial control of tissue morphology and cell fate in culture on the micron scale. Culture substrates presenting grafted poly(ethylene glycol) (PEG) brushes can be used to achieve this task by creating microscale, non-fouling and cell adhesion resistant regions as well as regions where cells participate in biospecific interactions with covalently tethered ligands. To engineer complex tissues using such substrates, it will be necessary to sequentially pattern multiple PEG brushes functionalized to confer differential bioactivities and aligned in microscale orientations that mimic in vivo niches. Microcontact printing (μCP) is a versatile technique to pattern such grafted PEG brushes, but manual μCP cannot be performed with microscale precision. Thus, we combined advanced robotics with soft-lithography techniques and emerging surface chemistry reactions to develop a robotic microcontact printing (R-μCP)-assisted method for fabricating culture substrates with complex, microscale, and highly ordered patterns of PEG brushes presenting orthogonal 'click' chemistries. Here, we describe in detail the workflow to manufacture such substrates.
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