Evidence map›Paper›PMID 25406832›Full record

ArticleNature communications2014

Positron emission tomography and functional characterization of a complete PBR/TSPO knockout.

Richard B Banati, Ryan J Middleton, Ronald Chan, Claire R Hatty, Winnie Wai-Ying Kam, Candice Quin, Manuel B Graeber, Arvind Parmar, David Zahra, Paul Callaghan and 7 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 145 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
145citing papers in PubMed, 2 pooled it
12.1field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

145 citing papers in PubMed, 2 syntheses or guidelines pooled it, 238 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
  3. The Biochemical journal · 2017
    Trial
  4. Review
  5. Article
  6. [BMC neurology · 2026
    Article
  7. Article
  8. Review
  9. Article
  10. STAR/STARD1: A mitochondrial intermembrane space cholesterol shuttle degraded through mitophagy.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Cholesterol-dependent enzyme activity of human TSPO1.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  16. Review
  17. Article
  18. Article
  19. Article
  20. Article

85 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors at 2 institutions in 2 countries.

Richard B Banati1] Life Sciences, Australian Nuclear Science and Technology Organisation, Locked Bag 2001, Kirrawee DC, New South Wales 2232, Australia [2] Brain &Mind Research Institute, The University of Sydney, Sydney, New South Wales 2006, Australia [3] Medical Imaging &Radiation Sciences, Faculty of Health Science and Brain &Mind Research Institute, The University of Sydney, Sydney, New South Wales 2006, Australia [4] National Imaging Facility, Sydney, Camperdown, New South Wales 2006, Australia.
Ryan J MiddletonLife Sciences, Australian Nuclear Science and Technology Organisation, Locked Bag 2001, Kirrawee DC, New South Wales 2232, Australia.
Ronald Chan1] Brain &Mind Research Institute, The University of Sydney, Sydney, New South Wales 2006, Australia [2] Medical Imaging &Radiation Sciences, Faculty of Health Science and Brain &Mind Research Institute, The University of Sydney, Sydney, New South Wales 2006, Australia.
Claire R Hatty1] Brain &Mind Research Institute, The University of Sydney, Sydney, New South Wales 2006, Australia [2] Medical Imaging &Radiation Sciences, Faculty of Health Science and Brain &Mind Research Institute, The University of Sydney, Sydney, New South Wales 2006, Australia.
Winnie Wai-Ying Kam1] Life Sciences, Australian Nuclear Science and Technology Organisation, Locked Bag 2001, Kirrawee DC, New South Wales 2232, Australia [2] Brain &Mind Research Institute, The University of Sydney, Sydney, New South Wales 2006, Australia [3] Medical Imaging &Radiation Sciences, Faculty of Health Science and Brain &Mind Research Institute, The University of Sydney, Sydney, New South Wales 2006, Australia.
Candice Quin1] Brain &Mind Research Institute, The University of Sydney, Sydney, New South Wales 2006, Australia [2] Medical Imaging &Radiation Sciences, Faculty of Health Science and Brain &Mind Research Institute, The University of Sydney, Sydney, New South Wales 2006, Australia.
Manuel B Graeber1] Brain &Mind Research Institute, The University of Sydney, Sydney, New South Wales 2006, Australia [2] Medical Imaging &Radiation Sciences, Faculty of Health Science and Brain &Mind Research Institute, The University of Sydney, Sydney, New South Wales 2006, Australia [3] Sydney Medical School, The University of Sydney, Sydney, New South Wales 2006, Australia.
Arvind ParmarLife Sciences, Australian Nuclear Science and Technology Organisation, Locked Bag 2001, Kirrawee DC, New South Wales 2232, Australia.
David ZahraLife Sciences, Australian Nuclear Science and Technology Organisation, Locked Bag 2001, Kirrawee DC, New South Wales 2232, Australia.
Paul CallaghanLife Sciences, Australian Nuclear Science and Technology Organisation, Locked Bag 2001, Kirrawee DC, New South Wales 2232, Australia.
Sandra FokBrain &Mind Research Institute, The University of Sydney, Sydney, New South Wales 2006, Australia.
Nicholas R HowellLife Sciences, Australian Nuclear Science and Technology Organisation, Locked Bag 2001, Kirrawee DC, New South Wales 2232, Australia.
Marie GregoireLife Sciences, Australian Nuclear Science and Technology Organisation, Locked Bag 2001, Kirrawee DC, New South Wales 2232, Australia.
Alexander Szabo1] Life Sciences, Australian Nuclear Science and Technology Organisation, Locked Bag 2001, Kirrawee DC, New South Wales 2232, Australia [2] Centre for Translational Neuroscience, University of Wollongong, Wollongong, New South Wales 2522, Australia.
Tien PhamLife Sciences, Australian Nuclear Science and Technology Organisation, Locked Bag 2001, Kirrawee DC, New South Wales 2232, Australia.
Emma DavisLife Sciences, Australian Nuclear Science and Technology Organisation, Locked Bag 2001, Kirrawee DC, New South Wales 2232, Australia.
Guo-Jun Liu1] Life Sciences, Australian Nuclear Science and Technology Organisation, Locked Bag 2001, Kirrawee DC, New South Wales 2232, Australia [2] Brain &Mind Research Institute, The University of Sydney, Sydney, New South Wales 2006, Australia [3] Medical Imaging &Radiation Sciences, Faculty of Health Science and Brain &Mind Research Institute, The University of Sydney, Sydney, New South Wales 2006, Australia.
Australian Nuclear Science and Technology Organisation · AUThe University of Sydney · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The evolutionarily conserved peripheral benzodiazepine receptor (PBR), or 18-kDa translocator protein (TSPO), is thought to be essential for cholesterol transport and steroidogenesis, and thus life. TSPO has been proposed as a biomarker of neuroinflammation and a new drug target in neurological diseases ranging from Alzheimer's disease to anxiety. Here we show that global C57BL/6-Tspo(tm1GuWu(GuwiyangWurra))-knockout mice are viable with normal growth, lifespan, cholesterol transport, blood pregnenolone concentration, protoporphyrin IX metabolism, fertility and behaviour. However, while the activation of microglia after neuronal injury appears to be unimpaired, microglia from (GuwiyangWurra)TSPO knockouts produce significantly less ATP, suggesting reduced metabolic activity. Using the isoquinoline PK11195, the ligand originally used for the pharmacological and structural characterization of the PBR/TSPO, and the imidazopyridines CLINDE and PBR111, we demonstrate the utility of (GuwiyangWurra)TSPO knockouts to provide robust data on drug specificity and selectivity, both in vitro and in vivo, as well as the mechanism of action of putative TSPO-targeting drugs.

Indexed as

Adenosine TriphosphateAdrenal GlandsAnimalsBehavior, AnimalBrainCholesterolFertilityKidneyMaleMiceMice, Inbred C57BLMice, KnockoutMicrogliaPositron-Emission TomographyPregnenoloneProtoporphyrinsAdenosine TriphosphateBzrp protein, mouseCholesterolPregnenoloneprotoporphyrin IXProtoporphyrinsReceptors, GABA

Identifiers

PMID25406832
PMCPMC4263137
OpenAlexW1986304241

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.