ArticleDevelopment (Cambridge, England)2014
RBPJ in mouse Sertoli cells is required for proper regulation of the testis stem cell niche.
Article in Development (Cambridge, England), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 59 citations in OpenAlex.
- Single-cell atlas of rhesus monkey testis reveals aging- and season-dependent regulation of spermatogenesis and microenvironment homeostasis.International journal of biological sciences · 2026Article
- Microenvironment of spermatogonial stem cells: a key factor in the regulation of spermatogenesis.Stem cell research & therapy · 2024Review
- STUB1 directs FOXQ1-mediated transactivation of Ldha gene and facilitates lactate production in mouse Sertoli cells.Cell and tissue research · 2023Article
- Sertoli cells are the source of stem cell factor for spermatogenesis.Development (Cambridge, England) · 2023Article
- Multi-breed genomic predictions and functional variants for fertility of tropical bulls.PloS one · 2023Article
- Regulation of spermatogonial stem cell self-renewal and proliferation in mammals.Histology and histopathology · 2022Review
- Nuclear and Membrane Receptors for Sex Steroids Are Involved in the Regulation of Delta/Serrate/LAG-2 Proteins in Rodent Sertoli Cells.International journal of molecular sciences · 2022Article
- Sertoli Cell-Germ Cell Interactions Within the Niche: Paracrine and Juxtacrine Molecular Communications.Frontiers in endocrinology · 2022Review
- Sumoylation and its regulation in testicular Sertoli cells.Biochemical and biophysical research communications · 2021Article
- CellCall: integrating paired ligand-receptor and transcription factor activities for cell-cell communication.Nucleic acids research · 2021Article
- CEP164 is essential for efferent duct multiciliogenesis and male fertility.Reproduction (Cambridge, England) · 2021Article
- E2F1 regulates testicular descent and controls spermatogenesis by influencing WNT4 signaling.Development (Cambridge, England) · 2021Article
- A framework for high-resolution phenotyping of candidate male infertility mutants: from human to mouse.Human genetics · 2021Review
- An Integrated Analysis of Network Pharmacology, Molecular Docking, and Experiment Validation to Explore the New Candidate Active Component and Mechanism ofDrug design, development and therapy · 2021Article
- WTAP Function in Sertoli Cells Is Essential for Sustaining the Spermatogonial Stem Cell Niche.Stem cell reports · 2020Article
- Characterization of rodent Sertoli cell primary cultures.Molecular reproduction and development · 2020Review
- Disruption of androgen signaling during puberty affects Notch pathway in rat seminiferous epithelium.Reproductive biology and endocrinology : RB&E · 2020Article
- Activation of Notch Signaling by Oocytes and Jag1 in Mouse Ovarian Granulosa Cells.Endocrinology · 2019Article
- Multiple signaling pathways in Sertoli cells: recent findings in spermatogenesis.Cell death & disease · 2019Review
- Undifferentiated spermatogonia regulateFASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Stem cells are influenced by their surrounding microenvironment, or niche. In the testis, Sertoli cells are the key niche cells directing the population size and differentiation fate of spermatogonial stem cells (SSCs). Failure to properly regulate SSCs leads to infertility or germ cell hyperplasia. Several Sertoli cell-expressed genes, such as Gdnf and Cyp26b1, have been identified as being indispensable for the proper maintenance of SSCs in their niche, but the pathways that modulate their expression have not been identified. Although we have recently found that constitutively activating NOTCH signaling in Sertoli cells leads to premature differentiation of all prospermatogonia and sterility, suggesting that there is a crucial role for this pathway in the testis stem cell niche, a true physiological function of NOTCH signaling in Sertoli cells has not been demonstrated. To this end, we conditionally ablated recombination signal binding protein for immunoglobulin kappa J region (Rbpj), a crucial mediator of NOTCH signaling, in Sertoli cells using Amh-cre. Rbpj knockout mice had: significantly increased testis sizes; increased expression of niche factors, such as Gdnf and Cyp26b1; significant increases in the number of pre- and post-meiotic germ cells, including SSCs; and, in a significant proportion of mice, testicular failure and atrophy with tubule lithiasis, possibly due to these unsustainable increases in the number of germ cells. We also identified germ cells as the NOTCH ligand-expressing cells. We conclude that NOTCH signaling in Sertoli cells is required for proper regulation of the testis stem cell niche and is a potential feedback mechanism, based on germ cell input, that governs the expression of factors that control SSC proliferation and differentiation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.