Evidence map›Paper›PMID 25406120›Full record

ArticlePlant physiology2015

The last step in cocaine biosynthesis is catalyzed by a BAHD acyltransferase.

Gregor Wolfgang Schmidt, Jan Jirschitzka, Tiffany Porta, Michael Reichelt, Katrin Luck, José Carlos Pardo Torre, Franziska Dolke, Emmanuel Varesio, Gérard Hopfgartner, Jonathan Gershenzon and 1 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Plant physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.

0numbers the graph read from it
0cells of the map it votes in
25citing papers in PubMed
2.0field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

25 citing papers in PubMed, 68 citations in OpenAlex.

  1. Review
  2. Review
  3. Review
  4. Article
  5. Genomic and structural basis for evolution of tropane alkaloid biosynthesis.Proceedings of the National Academy of Sciences of the United States of America · 2023
    Article
  6. Understanding how plants produce cocaine.Proceedings of the National Academy of Sciences of the United States of America · 2023
    Article
  7. Elucidation of tropane alkaloid biosynthesis inProceedings of the National Academy of Sciences of the United States of America · 2022
    Article
  8. Chemical Synthesis ofMolecules (Basel, Switzerland) · 2022
    Article
  9. Article
  10. Review
  11. Advances in chemistry and bioactivity of the genus Erythroxylum.Natural products and bioprospecting · 2022
    Review
  12. Review
  13. Review
  14. Article
  15. Article
  16. Article
  17. Article
  18. Review
  19. Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 2 countries.

Gregor Wolfgang SchmidtDepartment of Biochemistry, Max Planck Institute for Chemical Ecology, Beutenberg Campus, D-07745 Jena, Germany (G.W.S., J.J., M.R., K.L., J.C.P.T., F.D., J.G., J.C.D.); andLife Sciences Mass Spectrometry Research Unit, School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, 1211 Geneva, Switzerland (T.P., E.V., G.H.).
Jan JirschitzkaDepartment of Biochemistry, Max Planck Institute for Chemical Ecology, Beutenberg Campus, D-07745 Jena, Germany (G.W.S., J.J., M.R., K.L., J.C.P.T., F.D., J.G., J.C.D.); andLife Sciences Mass Spectrometry Research Unit, School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, 1211 Geneva, Switzerland (T.P., E.V., G.H.).ORCID http://orcid.org/0000-0003-1007-4700
Tiffany PortaDepartment of Biochemistry, Max Planck Institute for Chemical Ecology, Beutenberg Campus, D-07745 Jena, Germany (G.W.S., J.J., M.R., K.L., J.C.P.T., F.D., J.G., J.C.D.); andLife Sciences Mass Spectrometry Research Unit, School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, 1211 Geneva, Switzerland (T.P., E.V., G.H.).
Michael ReicheltDepartment of Biochemistry, Max Planck Institute for Chemical Ecology, Beutenberg Campus, D-07745 Jena, Germany (G.W.S., J.J., M.R., K.L., J.C.P.T., F.D., J.G., J.C.D.); andLife Sciences Mass Spectrometry Research Unit, School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, 1211 Geneva, Switzerland (T.P., E.V., G.H.).
Katrin LuckDepartment of Biochemistry, Max Planck Institute for Chemical Ecology, Beutenberg Campus, D-07745 Jena, Germany (G.W.S., J.J., M.R., K.L., J.C.P.T., F.D., J.G., J.C.D.); andLife Sciences Mass Spectrometry Research Unit, School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, 1211 Geneva, Switzerland (T.P., E.V., G.H.).
José Carlos Pardo TorreDepartment of Biochemistry, Max Planck Institute for Chemical Ecology, Beutenberg Campus, D-07745 Jena, Germany (G.W.S., J.J., M.R., K.L., J.C.P.T., F.D., J.G., J.C.D.); andLife Sciences Mass Spectrometry Research Unit, School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, 1211 Geneva, Switzerland (T.P., E.V., G.H.).
Franziska DolkeDepartment of Biochemistry, Max Planck Institute for Chemical Ecology, Beutenberg Campus, D-07745 Jena, Germany (G.W.S., J.J., M.R., K.L., J.C.P.T., F.D., J.G., J.C.D.); andLife Sciences Mass Spectrometry Research Unit, School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, 1211 Geneva, Switzerland (T.P., E.V., G.H.).
Emmanuel VaresioDepartment of Biochemistry, Max Planck Institute for Chemical Ecology, Beutenberg Campus, D-07745 Jena, Germany (G.W.S., J.J., M.R., K.L., J.C.P.T., F.D., J.G., J.C.D.); andLife Sciences Mass Spectrometry Research Unit, School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, 1211 Geneva, Switzerland (T.P., E.V., G.H.).
Gérard HopfgartnerDepartment of Biochemistry, Max Planck Institute for Chemical Ecology, Beutenberg Campus, D-07745 Jena, Germany (G.W.S., J.J., M.R., K.L., J.C.P.T., F.D., J.G., J.C.D.); andLife Sciences Mass Spectrometry Research Unit, School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, 1211 Geneva, Switzerland (T.P., E.V., G.H.).
Jonathan GershenzonDepartment of Biochemistry, Max Planck Institute for Chemical Ecology, Beutenberg Campus, D-07745 Jena, Germany (G.W.S., J.J., M.R., K.L., J.C.P.T., F.D., J.G., J.C.D.); andLife Sciences Mass Spectrometry Research Unit, School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, 1211 Geneva, Switzerland (T.P., E.V., G.H.).
John Charles D'AuriaDepartment of Biochemistry, Max Planck Institute for Chemical Ecology, Beutenberg Campus, D-07745 Jena, Germany (G.W.S., J.J., M.R., K.L., J.C.P.T., F.D., J.G., J.C.D.); andLife Sciences Mass Spectrometry Research Unit, School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, 1211 Geneva, Switzerland (T.P., E.V., G.H.) john.c.dauria@ttu.edu.ORCID http://orcid.org/0000-0002-4865-3938
University of Geneva · CHMax Planck Institute for Chemical Ecology · DEUniversity of Lausanne · CH

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The esterification of methylecgonine (2-carbomethoxy-3β-tropine) with benzoic acid is the final step in the biosynthetic pathway leading to the production of cocaine in Erythoxylum coca. Here we report the identification of a member of the BAHD family of plant acyltransferases as cocaine synthase. The enzyme is capable of producing both cocaine and cinnamoylcocaine via the activated benzoyl- or cinnamoyl-Coenzyme A thioesters, respectively. Cocaine synthase activity is highest in young developing leaves, especially in the palisade parenchyma and spongy mesophyll. These data correlate well with the tissue distribution pattern of cocaine as visualized with antibodies. Matrix-assisted laser-desorption ionization mass spectral imaging revealed that cocaine and cinnamoylcocaine are differently distributed on the upper versus lower leaf surfaces. Our findings provide further evidence that tropane alkaloid biosynthesis in the Erythroxylaceae occurs in the above-ground portions of the plant in contrast with the Solanaceae, in which tropane alkaloid biosynthesis occurs in the roots.

Indexed as

AcyltransferasesCatalysisCocaineErythroxylaceaeMesophyll CellsPlant LeavesPlant ProteinsAcyltransferasescinnamoylcocaineCocainePlant Proteins

Identifiers

PMID25406120
PMCPMC4281001
OpenAlexW2047781776

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.