ArticlePloS one2014
Discrimination of influenza infection (A/2009 H1N1) from prior exposure by antibody protein microarray analysis.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Mapping antibody footprints using binding profiles.Cell reports methods · 2023Article
- Article
- Estimating the cumulative incidence of SARS-CoV-2 with imperfect serological tests: Exploiting cutoff-free approaches.PLoS computational biology · 2021Article
- A Complex Dance: Measuring the Multidimensional Worlds of Influenza Virus Evolution and Anti-Influenza Immune Responses.Pathogens (Basel, Switzerland) · 2019Review
- Article
- Development of an influenza virus protein microarray to measure the humoral response to influenza virus infection in mallards.Emerging microbes & infections · 2017Article
- Infectious reactivation of cytomegalovirus explaining age- and sex-specific patterns of seroprevalence.PLoS computational biology · 2017Article
- Structure of general-population antibody titer distributions to influenza A virus.Scientific reports · 2017Article
- Novel multiplex assay platforms to detect influenza A hemagglutinin subtype-specific antibody responses for high-throughput and in-field applications.Influenza and other respiratory viruses · 2017Article
- Evaluation of multiplex assay platforms for detection of influenza hemagglutinin subtype specific antibody responses.Journal of virological methods · 2017Article
- Heterosubtypic cross-reactivity of HA1 antibodies to influenza A, with emphasis on nonhuman subtypes (H5N1, H7N7, H9N2).PloS one · 2017Article
- Quantifying homologous and heterologous antibody titre rises after influenza virus infection.Epidemiology and infection · 2016Article
- Joint modelling of serological and hospitalization data reveals that high levels of pre-existing immunity and school holidays shaped the influenza A pandemic of 2009 in the Netherlands.Journal of the Royal Society, Interface · 2015Article
- Correction: Discrimination of influenza infection (A/2009 H1N1) from prior exposure by antibody protein microarray analysis.PloS one · 2015Article
- Murine Monoclonal Antibodies for Antigenic Discrimination of HIV-1 Envelope Proteins.Viral immunologyArticle
Corrections and comments
- Erratum issued
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Reliable discrimination of recent influenza A infection from previous exposure using hemagglutination inhibition (HI) or virus neutralization tests is currently not feasible. This is due to low sensitivity of the tests and the interference of antibody responses generated by previous infections. Here we investigate the diagnostic characteristics of a newly developed antibody (HA1) protein microarray using data from cross-sectional serological studies carried out before and after the pandemic of 2009. The data are analysed by mixture models, providing a probabilistic classification of sera (susceptible, prior-exposed, recently infected). Estimated sensitivity and specificity for identifying A/2009 infections are low using HI (66% and 51%), and high when using A/2009 microarray data alone or together with A/1918 microarray data (96% and 95%). As a heuristic, a high A/2009 to A/1918 antibody ratio (>1.05) is indicative of recent infection, while a low ratio is indicative of a pre-existing response, even if the A/2009 titer is high. We conclude that highly sensitive and specific classification of individual sera is possible using the protein microarray, thereby enabling precise estimation of age-specific infection attack rates in the population even if sample sizes are small.
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