ArticlePloS one2014
Amorphous calcium carbonate precipitation by cellular biomineralization in mantle cell cultures of Pinctada fucata.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 62 citations in OpenAlex.
- Development of long-term oyster explant cultures enable sustained tissue-specific activity and reveal novel cellular behaviours.Biochemistry and biophysics reports · 2026Article
- Multi-tissue metabolomic profiling reveals the crucial metabolites and pathways associated with scallop growth.BMC genomics · 2024Article
- SlugAtlas, a histological and 3D online resource of the land slugs Deroceras laeve and Ambigolimax valentianus.PloS one · 2024Article
- Metabolic profiling of Mytilus coruscus mantle in response of shell repairing under acute acidification.PloS one · 2023Article
- Structure of an amorphous calcium carbonate phase involved in the formation ofProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Status in molluscan cell line development in last one decade (2010-2020): impediments and way forward.Cytotechnology · 2022Review
- Temperature-induced microstructural changes in shells of laboratory-grown Arctica islandica (Bivalvia).PloS one · 2021Article
- The calcitonin-like system is an ancient regulatory system of biomineralization.Scientific reports · 2020Article
- 3D Chitin Scaffolds of Marine Demosponge Origin for Biomimetic Mollusk Hemolymph-Associated BiomineralizationMarine drugs · 2020Article
- Dilution of Seawater Affects the CaFrontiers in physiology · 2020Article
- Calcium transfer across the outer mantle epithelium in the Pacific oyster,Proceedings. Biological sciences · 2018Article
- Cloning, characterization and functional analysis of an Alveoline-like protein in the shell of Pinctada fucata.Scientific reports · 2018Article
- Nacre formation by epithelial cell cultures from mantle of the black-lip pearl oyster, Pinctada margaritifera.In vitro cellular & developmental biology. Animal · 2018Article
- Biochemical characterization of the native α-carbonic anhydrase purified from the mantle of the Mediterranean mussel, Mytilus galloprovincialis.Journal of enzyme inhibition and medicinal chemistry · 2017Article
- Production and covalent immobilisation of the recombinant bacterial carbonic anhydrase (SspCA) onto magnetic nanoparticles.Journal of enzyme inhibition and medicinal chemistry · 2017Article
- The receptor genes PfBMPR1B and PfBAMBI are involved in regulating shell biomineralization in the pearl oyster Pinctada fucata.Scientific reports · 2017Article
- Characterization of an Alpha Type Carbonic Anhydrase from Paracentrotus lividus Sea Urchin Embryos.Marine biotechnology (New York, N.Y.) · 2016Article
- Transcriptome and biomineralization responses of the pearl oyster Pinctada fucata to elevated CO2 and temperature.Scientific reports · 2016Article
- In-depth proteomic analysis of shell matrix proteins of Pinctada fucata.Scientific reports · 2015Article
- Growth and regrowth of adult sea urchin spines involve hydrated and anhydrous amorphous calcium carbonate precursors.Journal of structural biology: XArticle
Corrections and comments
- Erratum issuedSu, Jingtan [corrected to Su, Jing-Tan]; Zhang, Guiyou [corrected to Zhang, Gui-You]; Xie, Liping [corrected to Xie, Li-Ping]; Zhang, Rongqing [corrected to Zhang, Rong-Qing]
Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The growth of molluscan shell crystals is generally thought to be initiated from the extrapallial fluid by matrix proteins, however, the cellular mechanisms of shell formation pathway remain unknown. Here, we first report amorphous calcium carbonate (ACC) precipitation by cellular biomineralization in primary mantle cell cultures of Pinctada fucata. Through real-time PCR and western blot analyses, we demonstrate that mantle cells retain the ability to synthesize and secrete ACCBP, Pif80 and nacrein in vitro. In addition, the cells also maintained high levels of alkaline phosphatase and carbonic anhydrase activity, enzymes responsible for shell formation. On the basis of polarized light microscopy and scanning electron microscopy, we observed intracellular crystals production by mantle cells in vitro. Fourier transform infrared spectroscopy and X-ray diffraction analyses revealed the crystals to be ACC, and de novo biomineralization was confirmed by following the incorporation of Sr into calcium carbonate. Our results demonstrate the ability of mantle cells to perform fundamental biomineralization processes via amorphous calcium carbonate, and these cells may be directly involved in pearl oyster shell formation.
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