ArticleFrontiers in cellular neuroscience2014
Atypical "seizure-like" activity in cortical reverberating networks in vitro can be caused by LPS-induced inflammation: a multi-electrode array study from a hundred neurons.
Article in Frontiers in cellular neuroscience, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 23 citations in OpenAlex.
- Minute-Resolution Sampling Reveals Rapid and Stimulus-Specific IL-1β Dynamics During Acute Epileptiform Activity.Neurochemical research · 2026Article
- Direct effects of prolonged TNF-α and IL-6 exposure on neural activity in human iPSC-derived neuron-astrocyte co-cultures.Frontiers in cellular neuroscience · 2025Article
- Visualization of incrementally learned projection trajectories for longitudinal data.Scientific reports · 2024Article
- From the Bush to the Brain: Preclinical Stages of Ethnobotanical Anti-Inflammatory and Neuroprotective Drug Discovery-An Australian Example.International journal of molecular sciences · 2023Review
- Sepsis-Induced Changes in Spectral Segregation and Kinetics of Hippocampal Oscillatory States in Rats.eNeuro · 2023Article
- Palmitoylethanolamide Modulation of Microglia Activation: Characterization of Mechanisms of Action and Implication for Its Neuroprotective Effects.International journal of molecular sciences · 2021Article
- Presynaptic L-Type CaThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Article
- Immune Challenges and Seizures: How Do Early Life Insults Influence Epileptogenesis?Frontiers in pharmacology · 2020Review
- Inflammatory cytokine-induced changes in neural network activity measured by waveform analysis of high-content calcium imaging in murine cortical neurons.Scientific reports · 2017Article
- Plant Polyphenols and Exendin-4 Prevent Hyperactivity and TNF-α Release in LPS-TreatedFrontiers in neuroscience · 2017Article
- Pharmacological Tools to Activate Microglia and their Possible use to Study Neural Network Patho-physiology.Current neuropharmacology · 2017Review
- Neuron-glia cross talk revealed in reverberating networks by simultaneous extracellular recording of spikes and astrocytes' glutamate transporter and K+ currents.Journal of neurophysiology · 2016Article
- Effective Reduction of Acute Ethanol Withdrawal by the Tetracycline Derivative, Tigecycline, in Female and Male DBA/2J Mice.Alcoholism, clinical and experimental research · 2016Article
- Leucine-Rich Repeat Kinase 2 Influences Fate Decision of Human Monocytes Differentiated from Induced Pluripotent Stem Cells.PloS one · 2016Article
- Commentary: "Comparison of spike parameters from optically identified GABAergic and glutamatergic neurons in sparse cortical cultures".Frontiers in cellular neuroscience · 2015Article
- Interaction of electrically evoked activity with intrinsic dynamics of cultured cortical networks with and without functional fast GABAergic synaptic transmission.Frontiers in cellular neuroscience · 2015Article
- Editorial: Mechanisms of neuroinflammation and inflammatory neurodegeneration in acute brain injury.Frontiers in cellular neuroscience · 2015Article
- Round Up the Unusual Suspects: Can Noninflammatory Microglia Drive Epileptogenesis?Epilepsy currentsArticle
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We show here that a mild sterile inflammation induced by the endotoxin lipopolysaccharide (LPS), in a neuron/astrocyte/microglial cortical network, modulates neuronal excitability and can initiate long-duration burst events resembling epileptiform seizures, a recognized feature of various central nervous neurodegenerative, neurological and acute systemic diseases associated with neuroinflammation. To study this action, we simultaneously analyzed the reverberating bursting activity of a hundred neurons by using in vitro multi-electrode array methods. ∼5 h after LPS application, we observed a net increase in the average number of spikes elicited in engaged cells and within each burst, but no changes neither in spike waveforms nor in burst rate. This effect was characterized by a slow, twofold exponential increase of the burst duration and the appearance of rarely occurring long burst events that were never seen during control recordings. These changes and the time-course of microglia-released proinflammatory cytokine, tumor necrosis factor-alpha (TNF-α), were blocked by pre-treatment with 50 nM minocycline, an established anti-inflammatory agent which was inactive when applied alone. Assay experiments also revealed that application of 60 pM exogenous TNF-α after 12-15 h, produced non-washable changes of neuronal excitability, completely different from those induced by LPS, suggesting that TNF-α release alone was not responsible for our observed findings. Our results indicate that the link between neuroinflammation and hyperexcitability can be unveiled by studying the long-term activity of in vitro neuronal/astrocyte/microglial networks.
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