ArticleJournal of bacteriology2015
Staphylococcus epidermidis SrrAB regulates bacterial growth and biofilm formation differently under oxic and microaerobic conditions.
Article in Journal of bacteriology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
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- Metabolic and regulatory adaptations of Staphylococcus epidermidis to host stresses.FEMS microbiology reviews · 2026Review
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- The pathogenicity and virulence of the opportunistic pathogenVirulence · 2024Review
- The Complex Intracellular Lifecycle ofInternational journal of molecular sciences · 2024Review
- Spectral characterization of a blue light-emitting micro-LED platform on skin-associated microbial chromophores.Biomedical optics express · 2024Article
- Illuminating microflora: shedding light on the potential of blue light to modulate the cutaneous microbiome.Frontiers in cellular and infection microbiology · 2024Review
- SarZ inhibits the hemolytic activity through regulation of phenol soluble modulins inFrontiers in cellular and infection microbiology · 2024Article
- Interactions of Neutrophils with the Polymeric Molecular Components of the Biofilm Matrix in the Context of Implant-Associated Bone and Joint Infections.International journal of molecular sciences · 2023Review
- The Vancomycin Resistance-Associated Regulatory System VraSR Modulates Biofilm Formation of Staphylococcus epidermidis in anmSphere · 2021Article
- Staphylococcal Biofilm Development: Structure, Regulation, and Treatment Strategies.Microbiology and molecular biology reviews : MMBR · 2020Review
- The SrrAB two-component system regulatesProceedings of the National Academy of Sciences of the United States of America · 2020Article
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- Monoclonal Antibodies Specific to the Extracellular Domain of Histidine Kinase YycG ofFrontiers in microbiology · 2020Article
- Management and Microbiological Characteristics of Membrane Formation on a Hydrophilic Acrylic Intraocular Lens: A Clinical Case Series and Material Comparative Study of Different IOLs.Journal of ophthalmology · 2019Article
- Transcriptomic Adjustments ofFrontiers in microbiology · 2019Article
- A Novel Antimicrobial Coating Represses Biofilm and Virulence-Related Genes in Methicillin-ResistantFrontiers in microbiology · 2018Article
- Nicotine EnhancesFrontiers in microbiology · 2018Article
- A Systematic Evaluation of the Two-Component Systems Network Reveals That ArlRS Is a Key Regulator of Catheter Colonization byFrontiers in microbiology · 2018Article
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11 authors.
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Abstract
SrrAB expression in Staphylococcus epidermidis strain 1457 (SE1457) was upregulated during a shift from oxic to microaerobic conditions. An srrA deletion (ΔsrrA) mutant was constructed for studying the regulatory function of SrrAB. The deletion resulted in retarded growth and abolished biofilm formation both in vitro and in vivo and under both oxic and microaerobic conditions. Associated with the reduced biofilm formation, the ΔsrrA mutant produced much less polysaccharide intercellular adhesion (PIA) and showed decreased initial adherence capacity. Microarray analysis showed that the srrA mutation affected transcription of 230 genes under microaerobic conditions, and 51 genes under oxic conditions. Quantitative real-time PCR confirmed this observation and showed downregulation of genes involved in maintaining the electron transport chain by supporting cytochrome and quinol-oxidase assembly (e.g., qoxB and ctaA) and in anaerobic metabolism (e.g., pflBA and nrdD). In the ΔsrrA mutant, the expression of the biofilm formation-related gene icaR was upregulated under oxic conditions and downregulated under microaerobic conditions, whereas icaA was downregulated under both conditions. An electrophoretic mobility shift assay further revealed that phosphorylated SrrA bound to the promoter regions of icaR, icaA, qoxB, and pflBA, as well as its own promoter region. These findings demonstrate that in S. epidermidis SrrAB is an autoregulator and regulates biofilm formation in an ica-dependent manner. Under oxic conditions, SrrAB modulates electron transport chain activity by positively regulating qoxBACD transcription. Under microaerobic conditions, it regulates fermentation processes and DNA synthesis by modulating the expression of both the pfl operon and nrdDG.
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