ArticleProceedings of the National Academy of Sciences of the United States of America2014
Fast high-resolution 3D total internal reflection fluorescence microscopy by incidence angle scanning and azimuthal averaging.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 78 citations in OpenAlex.
- Photonic lantern TIRF microscopy for highly efficient, uniform, artifact-free imaging.Optics express · 2024Article
- Imaging nanoscale axial dynamics at the basal plasma membrane.The international journal of biochemistry & cell biology · 2023Review
- SPITFIR(e): a supermaneuverable algorithm for fast denoising and deconvolution of 3D fluorescence microscopy images and videos.Scientific reports · 2023Article
- A generative model to simulate spatiotemporal dynamics of biomolecules in cells.Biological imaging · 2023Article
- BayesTICS: Local temporal image correlation spectroscopy and Bayesian simulation technique for sparse estimation of diffusion in fluorescence imaging.Biological imaging · 2023Article
- Fast widefield scan provides tunable and uniform illumination optimizing super-resolution microscopy on large fields.Nature communications · 2021Article
- STochastic Optical Reconstruction Microscopy (STORM) reveals the nanoscale organization of pathological aggregates in human brain.Neuropathology and applied neurobiology · 2021Article
- Three-dimensional total-internal reflection fluorescence nanoscopy with nanometric axial resolution by photometric localization of single molecules.Nature communications · 2021Article
- Single-shot, shadowless total internal reflection fluorescence microscopy via annular fiber bundle.Optics letters · 2020Article
- Variable incidence angle linear dichroism (VALiD): a technique for unique 3D orientation measurement of fluorescent ensembles.Optics express · 2020Article
- Calibrating Evanescent-Wave Penetration Depths for Biological TIRF Microscopy.Biophysical journal · 2019Review
- VIEW-MOD: a versatile illumination engine with a modular optical design for fluorescence microscopy.Optics express · 2019Article
- RAB6 and microtubules restrict protein secretion to focal adhesions.The Journal of cell biology · 2019Article
- E-syt1 Re-arranges STIM1 Clusters to Stabilize Ring-shaped ER-PM Contact Sites and Accelerate CaScientific reports · 2019Article
- Label-free surface-sensitive photonic microscopy with high spatial resolution using azimuthal rotation illumination.Science advances · 2019Article
- One-step deconvolution for multi-angle TIRF microscopy with enhanced resolution.Biomedical optics express · 2019Article
- Nanometric axial resolution of fibronectin assembly units achieved with an efficient reconstruction approach for multi-angle-TIRF microscopy.Scientific reports · 2019Article
- Multi-color live-cell super-resolution volume imaging with multi-angle interference microscopy.Nature communications · 2018Article
- Article
- In Vivo Single-Molecule Tracking at the Drosophila Presynaptic Motor Nerve Terminal.Journal of visualized experiments : JoVE · 2018Article
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Total internal reflection fluorescence microscopy (TIRFM) is the method of choice to visualize a variety of cellular processes in particular events localized near the plasma membrane of live adherent cells. This imaging technique not relying on particular fluorescent probes provides a high sectioning capability. It is, however, restricted to a single plane. We present here a method based on a versatile design enabling fast multiwavelength azimuthal averaging and incidence angles scanning to computationally reconstruct 3D images sequences. We achieve unprecedented 50-nm axial resolution over a range of 800 nm above the coverslip. We apply this imaging modality to obtain structural and dynamical information about 3D actin architectures. We also temporally decipher distinct Rab11a-dependent exocytosis events in 3D at a rate of seven stacks per second.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.