ArticleProceedings of the National Academy of Sciences of the United States of America2014
Global structural changes of an ion channel during its gating are followed by ion mobility mass spectrometry.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed.
- Review
- Diethylpyrocarbonate-Based Covalent Labeling Mass Spectrometry of Protein Interactions in a Membrane Complex System.Journal of the American Society for Mass Spectrometry · 2023Article
- Approaches for the modulation of mechanosensitive MscL channel pores.Frontiers in chemistry · 2023Review
- Investigating the Lipid Selectivity of Membrane Proteins in Heterogeneous Nanodiscs.Analytical chemistry · 2022Article
- Pocket delipidation induced by membrane tension or modification leads to a structurally analogous mechanosensitive channel state.Structure (London, England : 1993) · 2022Article
- Cycloalkane-modified amphiphilic polymers provide direct extraction of membrane proteins for CryoEM analysis.Communications biology · 2021Article
- Native Mass Spectrometry of Membrane Proteins.Analytical chemistry · 2021Review
- Integrative Mass Spectrometry-Based Approaches for Modeling Macromolecular Assemblies.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Collision-Induced Unfolding Differentiates Functional Variants of the KCNQ1 Voltage Sensor Domain.Journal of the American Society for Mass Spectrometry · 2020Article
- Effects of Detergent on α-Synuclein Structure. A Native MS-Ion Mobility Study.International journal of molecular sciences · 2020Article
- In-Lipid Structure of Pressure-Sensitive Domains Hints Mechanosensitive Channel Functional Diversity.Biophysical journal · 2020Article
- Current Methods for Detecting Cell Membrane Transient Interactions.Frontiers in chemistry · 2020Review
- Interrogating Membrane Protein Structure and Lipid Interactions by Native Mass Spectrometry.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Integrating hydrogen-deuterium exchange mass spectrometry with molecular dynamics simulations to probe lipid-modulated conformational changes in membrane proteins.Nature protocols · 2019Article
- Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry.Journal of visualized experiments : JoVE · 2018Article
- Structural Lipids Enable the Formation of Functional Oligomers of the Eukaryotic Purine Symporter UapA.Cell chemical biology · 2018Article
- Charge Neutralization Drives the Shape Reconfiguration of DNA Nanotubes.Angewandte Chemie (International ed. in English) · 2018Article
- Identifying key membrane protein lipid interactions using mass spectrometry.Nature protocols · 2018Article
- Lipids Shape the Electron Acceptor-Binding Site of the Peripheral Membrane Protein Dihydroorotate Dehydrogenase.Cell chemical biology · 2018Article
- Structural Dynamics of the MscL C-terminal Domain.Scientific reports · 2017Article
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9 authors.
Funding
Abstract
Mechanosensitive ion channels are sensors probing membrane tension in all species; despite their importance and vital role in many cell functions, their gating mechanism remains to be elucidated. Here, we determined the conditions for releasing intact mechanosensitive channel of large conductance (MscL) proteins from their detergents in the gas phase using native ion mobility-mass spectrometry (IM-MS). By using IM-MS, we could detect the native mass of MscL from Escherichia coli, determine various global structural changes during its gating by measuring the rotationally averaged collision cross-sections, and show that it can function in the absence of a lipid bilayer. We could detect global conformational changes during MscL gating as small as 3%. Our findings will allow studying native structure of many other membrane proteins.
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