ArticleProceedings of the National Academy of Sciences of the United States of America2014
Structural model of an mRNA in complex with the bacterial chaperone Hfq.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 76 citations in OpenAlex.
- Article
- Effect ofInternational journal of microbiology · 2025Article
- Control of iron acquisition by multiple small RNAs unravels a new role for transcriptional terminator loops in gene regulation.Nucleic acids research · 2024Article
- RNA compaction and iterative scanning for small RNA targets by the Hfq chaperone.Nature communications · 2024Article
- Small-Angle X-Ray Scattering for Macromolecular Complexes.Advances in experimental medicine and biology · 2024Article
- Small RNAs and Hfq capture unfolded RNA target sites during transcription.Molecular cell · 2023Article
- Genetically encoded chemical crosslinking of RNA in vivo.Nature chemistry · 2023Article
- Dynamic Refolding of OxyS sRNA by the Hfq RNA Chaperone.Journal of molecular biology · 2022Article
- Article
- Bacterial RNA chaperones and chaperone-like riboregulators: behind the scenes of RNA-mediated regulation of cellular metabolism.RNA biology · 2022Article
- The Amyloid Region of Hfq Riboregulator Promotes DsrA:Biology · 2021Article
- Specific and Global RNA Regulators inInternational journal of molecular sciences · 2021Review
- Stepwise sRNA targeting of structured bacterial mRNAs leads to abortive annealing.Molecular cell · 2021Article
- Article
- Effects of individual base-pairs on in vivo target search and destruction kinetics of bacterial small RNA.Nature communications · 2021Article
- Impacts of Small RNAs and Their Chaperones on Bacterial Pathogenicity.Frontiers in cellular and infection microbiology · 2021Review
- Distinctive Regulation of Carbapenem Susceptibility inFrontiers in microbiology · 2020Article
- Article
- Hfq-dependent mRNA unfolding promotes sRNA-based inhibition of translation.The EMBO journal · 2019Article
- Conformation and mechanical property of rpoS mRNA inhibitory stem studied by optical tweezers and X-ray scattering.PloS one · 2019Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
The Sm-like protein Hfq (host factor Q-beta phage) facilitates regulation by bacterial small noncoding RNAs (sRNAs) in response to stress and other environmental signals. Here, we present a low-resolution model of Escherichia coli Hfq bound to the rpoS mRNA, a bacterial stress response gene that is targeted by three different sRNAs. Selective 2'-hydroxyl acylation and primer extension, small-angle X-ray scattering, and Monte Carlo molecular dynamics simulations show that the distal face and lateral rim of Hfq interact with three sites in the rpoS leader, folding the RNA into a compact tertiary structure. These interactions are needed for sRNA regulation of rpoS translation and position the sRNA target adjacent to an sRNA binding region on the proximal face of Hfq. Our results show how Hfq specifically distorts the structure of the rpoS mRNA to enable sRNA base pairing and translational control.
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