ArticleBMC plant biology2014
Cysteine protease and cystatin expression and activity during soybean nodule development and senescence.
Article in BMC plant biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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32 citing papers in PubMed, 79 citations in OpenAlex.
- ESN3, a nodule-specific small peptide essential for symbiotic nitrogen fixation in Medicago truncatula.The New phytologist · 2026Article
- Maintaining sulfur supply to the symbiosome delays nodule senescence in soybean.Nature communications · 2025Article
- Papain-like cysteine proteases inFrontiers in plant science · 2025Article
- A mitochondrial metalloprotease FtsH4 is required for symbiotic nitrogen fixation in Lotus japonicus nodules.Scientific reports · 2024Article
- Loss of Lateral suppressor gene is associated with evolution of root nodule symbiosis in Leguminosae.Genome biology · 2024Article
- Defense and senescence interplay in legume nodules.Plant communications · 2024Review
- Spatial co-transcriptomics reveals discrete stages of the arbuscular mycorrhizal symbiosis.Nature plants · 2024Article
- Effects of Elevated Temperature onInternational journal of molecular sciences · 2023Article
- GmNAC039 and GmNAC018 activate the expression of cysteine protease genes to promote soybean nodule senescence.The Plant cell · 2023Article
- Article
- Critical role for uricase and xanthine dehydrogenase in soybean nitrogen fixation and nodule development.The plant genome · 2023Article
- Soybean gene co-expression network analysis identifies two co-regulated gene modules associated with nodule formation and development.Molecular plant pathology · 2023Article
- Insight into the control of nodule immunity and senescence during Medicago truncatula symbiosis.Plant physiology · 2023Article
- Genome-wide association identifies candidate genes for drought tolerance in coast redwood and giant sequoia.The Plant journal : for cell and molecular biology · 2022Article
- Plant protease as regulator and signaling molecule for enhancing environmental stress-tolerance.Plant cell reports · 2021Review
- Characteristics and Research Progress of Legume Nodule Senescence.Plants (Basel, Switzerland) · 2021Review
- Transcriptional control of local auxin distribution by the CsDFB1-CsPHB module regulates floral organogenesis in cucumber.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Genome-wide survey of soybean papain-like cysteine proteases and their expression analysis in root nodule symbiosis.BMC plant biology · 2020Article
- Responses of mature symbiotic nodules to the whole-plant systemic nitrogen signaling.Journal of experimental botany · 2020Article
- The rhizobial autotransporter determines the symbiotic nitrogen fixation activity ofProceedings of the National Academy of Sciences of the United States of America · 2020Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundNodules play an important role in fixing atmospheric nitrogen for soybean growth. Premature senescence of nodules can negatively impact on nitrogen availability for plant growth and, as such, we need a better understanding of nodule development and senescence. Cysteine proteases are known to play a role in nodule senescence, but knowledge is still fragmented regarding the function their inhibitors (cystatins) during the development and senescence of soybean nodules. This study provides the first data with regard to cystatin expression during nodule development combined with biochemical characterization of their inhibition strength.
resultsSeventy nine non-redundant cysteine protease gene sequences with homology to papain, belonging to different subfamilies, and several legumain-like cysteine proteases (vacuole processing enzymes) were identified from the soybean genome assembly with eighteen of these cysteine proteases actively transcribed during nodule development and senescence. In addition, nineteen non-redundant cystatins similar to oryzacystatin-I and belonging to cystatin subgroups A and C were identified from the soybean genome assembly with seven actively transcribed in nodules. Most cystatins had preferential affinity to cathepsin L-like cysteine proteases. Transcription of cystatins Glyma05g28250, Glyma15g12211, Glyma15g36180 particularly increased during onset of senescence, possibly regulating proteolysis when nodules senesce and undergo programmed cell death. Both actively transcribed and non-actively transcribed nodule cystatins inhibited cathepsin-L- and B-like activities in different age nodules and they also inhibited papain and cathepsin-L activity when expressed and purified from bacterial cells.
conclusionsOverlap in activities and specificities of actively and non-actively transcribed cystatins raises the question if non-transcribed cystatins provide a reservoir for response to particular environments. This data might be applicable to the development of strategies to extend the active life span of nodules or prevent environmentally induced senescence.
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