ArticleThe American journal of tropical medicine and hygiene2015
Molecular differentiation of Treponema pallidum subspecies in skin ulceration clinically suspected as yaws in Vanuatu using real-time multiplex PCR and serological methods.
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Article in The American journal of tropical medicine and hygiene, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers, 1 of them a synthesis that pooled it.
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39citing papers in PubMed, 1 pooled it
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Who cites it
39 citing papers in PubMed, 1 synthesis or guideline pooled it, 69 citations in OpenAlex.
- Metaanalysis of the Performance of a Combined Treponemal and Nontreponemal Rapid Diagnostic Test for Syphilis and Yaws.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2016Pooled it
- A Class IInfection and immunity · 2019Trial
- Comparative efficacy of low-dose versus standard-dose azithromycin for patients with yaws: a randomised non-inferiority trial in Ghana and Papua New Guinea.The Lancet. Global health · 2018Trial
- Haemophilus ducreyi Bacteria Causing Cutaneous Ulcer Outbreak in Children, Sierra Leone, December 2025.Emerging infectious diseases · 2026Article
- Whole-Genome Analysis of Treponema pallidum Subspecies endemicum among Men Who Have Sex with Men, Japan, 2020-2023.Emerging infectious diseases · 2026Article
- Assessment of the role of Haemophilus ducreyi coinfection on outcomes of Yaws treatment in the southwestern part of Ghana.PLoS neglected tropical diseases · 2026Article
- Mapping the distribution of yaws and Haemophilus ducreyi in the western north region of Ghana.BMC infectious diseases · 2025Observational
- Yaws in Africa: Past, Present and Future.Diseases (Basel, Switzerland) · 2025Review
- Multiplex Loop-Mediated Isothermal Amplification Coupled Lateral Flow Assay for Point-of-Care Detection of Syphilis.Indian journal of microbiology · 2024Article
- CDC Laboratory Recommendations for Syphilis Testing, United States, 2024.MMWR. Recommendations and reports : Morbidity and mortality weekly report. Recommendations and reports · 2024Article
- Prevalence and risk factors associated with Haemophilus ducreyi cutaneous ulcers in Cameroon.PLoS neglected tropical diseases · 2023Article
- A Systematic Review on Suitability of Molecular Techniques for Diagnosis and Research into Infectious Diseases of Concern in Resource-Limited Settings.Current issues in molecular biology · 2022Review
- Article
- Diagnosing point-of-care diagnostics for neglected tropical diseases.PLoS neglected tropical diseases · 2021Review
- Ulcerative skin lesions among children in Cameroon: It is not always Yaws.PLoS neglected tropical diseases · 2021Article
- Multiplex Recombinase Polymerase Amplification Assay for Simultaneous Detection ofTropical medicine and infectious disease · 2020Article
- A Loop-Mediated Isothermal Amplification Assay for the Detection ofThe American journal of tropical medicine and hygiene · 2020Article
- Syphilis Laboratory Guidelines: Performance Characteristics of Nontreponemal Antibody Tests.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2020Review
- Endemicity of Yaws and Seroprevalence of Treponema pallidum Antibodies in Nonhuman Primates, Kenya.Emerging infectious diseases · 2019Article
- Directly Sequenced Genomes of Contemporary Strains of Syphilis Reveal Recombination-Driven Diversity in Genes Encoding Predicted Surface-Exposed Antigens.Frontiers in microbiology · 2019Article
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Authors and funding
12 authors at 1 institution in 1 country.
Kai-Hua ChiLaboratory Reference and Research Branch, Division of STD Prevention, National Center for HIV/AIDS, Viral Hepatitis, STD and Tuberculosis Prevention, Atlanta, Georgia; Centers for Disease Control and Prevention, Atlanta, Georgia; Ministry of Health, Port Vila, Vanuatu; Center for Global Health, Centers for Disease Control and Prevention, Atlanta, Georgia; World Health Organization, Port Vila, Vanuatu; World Health Organization Consultant, Springhill, Brisbane, Australia; Department of Control of Neglected Tropical Diseases, World Health Organization, Geneva, Switzerland; World Health Organization, Western Pacific Regional Office, Manila, Philippines.
Damien DanavallLaboratory Reference and Research Branch, Division of STD Prevention, National Center for HIV/AIDS, Viral Hepatitis, STD and Tuberculosis Prevention, Atlanta, Georgia; Centers for Disease Control and Prevention, Atlanta, Georgia; Ministry of Health, Port Vila, Vanuatu; Center for Global Health, Centers for Disease Control and Prevention, Atlanta, Georgia; World Health Organization, Port Vila, Vanuatu; World Health Organization Consultant, Springhill, Brisbane, Australia; Department of Control of Neglected Tropical Diseases, World Health Organization, Geneva, Switzerland; World Health Organization, Western Pacific Regional Office, Manila, Philippines.
Fasihah TaleoLaboratory Reference and Research Branch, Division of STD Prevention, National Center for HIV/AIDS, Viral Hepatitis, STD and Tuberculosis Prevention, Atlanta, Georgia; Centers for Disease Control and Prevention, Atlanta, Georgia; Ministry of Health, Port Vila, Vanuatu; Center for Global Health, Centers for Disease Control and Prevention, Atlanta, Georgia; World Health Organization, Port Vila, Vanuatu; World Health Organization Consultant, Springhill, Brisbane, Australia; Department of Control of Neglected Tropical Diseases, World Health Organization, Geneva, Switzerland; World Health Organization, Western Pacific Regional Office, Manila, Philippines.
Allan PillayLaboratory Reference and Research Branch, Division of STD Prevention, National Center for HIV/AIDS, Viral Hepatitis, STD and Tuberculosis Prevention, Atlanta, Georgia; Centers for Disease Control and Prevention, Atlanta, Georgia; Ministry of Health, Port Vila, Vanuatu; Center for Global Health, Centers for Disease Control and Prevention, Atlanta, Georgia; World Health Organization, Port Vila, Vanuatu; World Health Organization Consultant, Springhill, Brisbane, Australia; Department of Control of Neglected Tropical Diseases, World Health Organization, Geneva, Switzerland; World Health Organization, Western Pacific Regional Office, Manila, Philippines.
Tun YeLaboratory Reference and Research Branch, Division of STD Prevention, National Center for HIV/AIDS, Viral Hepatitis, STD and Tuberculosis Prevention, Atlanta, Georgia; Centers for Disease Control and Prevention, Atlanta, Georgia; Ministry of Health, Port Vila, Vanuatu; Center for Global Health, Centers for Disease Control and Prevention, Atlanta, Georgia; World Health Organization, Port Vila, Vanuatu; World Health Organization Consultant, Springhill, Brisbane, Australia; Department of Control of Neglected Tropical Diseases, World Health Organization, Geneva, Switzerland; World Health Organization, Western Pacific Regional Office, Manila, Philippines.
Eli NachamkinLaboratory Reference and Research Branch, Division of STD Prevention, National Center for HIV/AIDS, Viral Hepatitis, STD and Tuberculosis Prevention, Atlanta, Georgia; Centers for Disease Control and Prevention, Atlanta, Georgia; Ministry of Health, Port Vila, Vanuatu; Center for Global Health, Centers for Disease Control and Prevention, Atlanta, Georgia; World Health Organization, Port Vila, Vanuatu; World Health Organization Consultant, Springhill, Brisbane, Australia; Department of Control of Neglected Tropical Diseases, World Health Organization, Geneva, Switzerland; World Health Organization, Western Pacific Regional Office, Manila, Philippines.
Jacob L KoolLaboratory Reference and Research Branch, Division of STD Prevention, National Center for HIV/AIDS, Viral Hepatitis, STD and Tuberculosis Prevention, Atlanta, Georgia; Centers for Disease Control and Prevention, Atlanta, Georgia; Ministry of Health, Port Vila, Vanuatu; Center for Global Health, Centers for Disease Control and Prevention, Atlanta, Georgia; World Health Organization, Port Vila, Vanuatu; World Health Organization Consultant, Springhill, Brisbane, Australia; Department of Control of Neglected Tropical Diseases, World Health Organization, Geneva, Switzerland; World Health Organization, Western Pacific Regional Office, Manila, Philippines.
David FeganLaboratory Reference and Research Branch, Division of STD Prevention, National Center for HIV/AIDS, Viral Hepatitis, STD and Tuberculosis Prevention, Atlanta, Georgia; Centers for Disease Control and Prevention, Atlanta, Georgia; Ministry of Health, Port Vila, Vanuatu; Center for Global Health, Centers for Disease Control and Prevention, Atlanta, Georgia; World Health Organization, Port Vila, Vanuatu; World Health Organization Consultant, Springhill, Brisbane, Australia; Department of Control of Neglected Tropical Diseases, World Health Organization, Geneva, Switzerland; World Health Organization, Western Pacific Regional Office, Manila, Philippines.
Kingsley AsieduLaboratory Reference and Research Branch, Division of STD Prevention, National Center for HIV/AIDS, Viral Hepatitis, STD and Tuberculosis Prevention, Atlanta, Georgia; Centers for Disease Control and Prevention, Atlanta, Georgia; Ministry of Health, Port Vila, Vanuatu; Center for Global Health, Centers for Disease Control and Prevention, Atlanta, Georgia; World Health Organization, Port Vila, Vanuatu; World Health Organization Consultant, Springhill, Brisbane, Australia; Department of Control of Neglected Tropical Diseases, World Health Organization, Geneva, Switzerland; World Health Organization, Western Pacific Regional Office, Manila, Philippines.
Lasse S VestergaardLaboratory Reference and Research Branch, Division of STD Prevention, National Center for HIV/AIDS, Viral Hepatitis, STD and Tuberculosis Prevention, Atlanta, Georgia; Centers for Disease Control and Prevention, Atlanta, Georgia; Ministry of Health, Port Vila, Vanuatu; Center for Global Health, Centers for Disease Control and Prevention, Atlanta, Georgia; World Health Organization, Port Vila, Vanuatu; World Health Organization Consultant, Springhill, Brisbane, Australia; Department of Control of Neglected Tropical Diseases, World Health Organization, Geneva, Switzerland; World Health Organization, Western Pacific Regional Office, Manila, Philippines.
Ronald C BallardLaboratory Reference and Research Branch, Division of STD Prevention, National Center for HIV/AIDS, Viral Hepatitis, STD and Tuberculosis Prevention, Atlanta, Georgia; Centers for Disease Control and Prevention, Atlanta, Georgia; Ministry of Health, Port Vila, Vanuatu; Center for Global Health, Centers for Disease Control and Prevention, Atlanta, Georgia; World Health Organization, Port Vila, Vanuatu; World Health Organization Consultant, Springhill, Brisbane, Australia; Department of Control of Neglected Tropical Diseases, World Health Organization, Geneva, Switzerland; World Health Organization, Western Pacific Regional Office, Manila, Philippines.
Cheng-Yen ChenLaboratory Reference and Research Branch, Division of STD Prevention, National Center for HIV/AIDS, Viral Hepatitis, STD and Tuberculosis Prevention, Atlanta, Georgia; Centers for Disease Control and Prevention, Atlanta, Georgia; Ministry of Health, Port Vila, Vanuatu; Center for Global Health, Centers for Disease Control and Prevention, Atlanta, Georgia; World Health Organization, Port Vila, Vanuatu; World Health Organization Consultant, Springhill, Brisbane, Australia; Department of Control of Neglected Tropical Diseases, World Health Organization, Geneva, Switzerland; World Health Organization, Western Pacific Regional Office, Manila, Philippines cychen@cdc.gov.
Centers for Disease Control and Prevention · US
Funding
Intramural CDC HHS CC999999World Health Organization 001
Abstract
We developed a TaqMan-based real-time quadriplex polymerase chain reaction (PCR) to simultaneously detect Treponema pallidum subspecies pallidum, T. pallidum subsp. pertenue, and T. pallidum subsp. endemicum, the causative agents of venereal syphilis, yaws, and bejel, respectively. The PCR assay was applied to samples from skin ulcerations of clinically presumptive yaws cases among children on Tanna Island, Vanuatu. Another real-time triplex PCR was used to screen for the point mutations in the 23S rRNA genes that have previously been associated with azithromycin resistance in T. pallidum subsp. pallidum strains. Seropositivity by the classical syphilis serological tests was 35.5% among children with skin ulcerations clinically suspected with yaws, whereas the presence of T. pallidum subsp. pertenue DNA was only found in lesions from 15.5% of children. No evidence of T. pallidum subsp. pertenue infection, by either PCR or serology was found in ∼59% of cases indicating alternative causes of yaws-like lesions in this endemic area.
Indexed as
AdolescentAnti-Bacterial AgentsAzithromycinBase SequenceChildDNA PrimersHumansMultiplex Polymerase Chain ReactionPhilippinesReal-Time Polymerase Chain ReactionSkin UlcerTreponema pallidumYawsAnti-Bacterial AgentsAzithromycinDNA Primers
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.