Evidence map›Paper›PMID 25403753›Full record

ArticleMolecular systems biology2014

Rapid neurogenesis through transcriptional activation in human stem cells.

Volker Busskamp, Nathan E Lewis, Patrick Guye, Alex H M Ng, Seth L Shipman, Susan M Byrne, Neville E Sanjana, Jernej Murn, Yinqing Li, Shangzhong Li and 3 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular systems biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 157 papers.

0numbers the graph read from it
0cells of the map it votes in
157citing papers in PubMed
6.4field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

157 citing papers in PubMed, 255 citations in OpenAlex.

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  12. Temporal Transcriptional Regulation of Human Neuronal Differentiation via Forward Programming.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026
    Article
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  19. Molecular Visualization of Neuronal TDP43 PathologybioRxiv : the preprint server for biology · 2025
    Article
  20. HyperCas12a enables multiplexed CRISPRi screens.bioRxiv : the preprint server for biology · 2025
    Article

97 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 7 institutions in 2 countries.

Volker BusskampDepartment of Genetics, Harvard Medical School, Boston, MA, USA Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
Nathan E LewisDepartment of Genetics, Harvard Medical School, Boston, MA, USA Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA Department of Biology, Brigham Young University, Provo, UT, USA Department of Pediatrics, University of California, San Diego, CA, USA.
Patrick GuyeDepartment of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
Alex H M NgDepartment of Genetics, Harvard Medical School, Boston, MA, USA Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA Department of Systems Biology, Harvard Medical School, Boston, MA, USA.
Seth L ShipmanDepartment of Genetics, Harvard Medical School, Boston, MA, USA Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
Susan M ByrneDepartment of Genetics, Harvard Medical School, Boston, MA, USA Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
Neville E SanjanaBroad Institute of MIT and Harvard Cambridge Center, Cambridge, MA, USA McGovern Institute for Brain Research, Department of Brain and Cognitive Sciences, Department of Biological Engineering, Massachusetts Institute of Technology (MIT), Cambridge, MA, USA.
Jernej MurnDepartment of Cell Biology, Harvard Medical School, Boston, MA, USA Division of Newborn Medicine, Boston Children's Hospital, Boston, MA, USA.
Yinqing LiDepartment of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
Shangzhong LiDepartment of Bioengineering, University of California, San Diego, CA, USA.
Michael StadlerFriedrich Miescher Institute for Biomedical Research, Basel, Switzerland Swiss Institute of Bioinformatics, Basel, Switzerland University of Basel, Basel, Switzerland.
Ron WeissDepartment of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
George M ChurchDepartment of Genetics, Harvard Medical School, Boston, MA, USA Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA gchurch@genetics.med.harvard.edu.
Massachusetts Institute of Technology · USHarvard University · USWyss Institute for Biologically Inspired EngineeringBoston Children's Hospital · USCenter for Systems Biology · USLa Jolla Bioengineering Institute · USSIB Swiss Institute of Bioinformatics · CH

Funding

Causal Transcriptional Consequences of Human Genetic VariationP50HG005550 · NHGRI · HARVARD MEDICAL SCHOOL · PI CHURCH, GEORGE M · 2010 to 2014
$19.0M
Training in the Molecular Biology of Neurodegeneration and Alzheimer's DiseaseT32AG000222 · NIA · HARVARD UNIVERSITY (MEDICAL SCHOOL) · PI Bruce A YANKNER · 1992 to 2026
$18.5M
Genetic circuits for high-throughput, multi-sensory, live cell microRNA profR01CA173712 · NCI · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI WEISS, RON · 2013 to 2017
$2.5M
Genome Engineering an IPSC Model of Alzheimer's DiseaseRF1AG048056 · NIA · HARVARD MEDICAL SCHOOL · PI CHURCH, GEORGE M, YANKNER, BRUCE A · 2014 to 2014
$2.1M
NCI NIH HHS R01 CA173712NHGRI NIH HHS P50 HG005550NHGRI NIH HHS P50 HG0540879NHGRI NIH HHS P50 HG0939511NIA NIH HHS RF1 AG048056NIA NIH HHS T32 AG000222
6 · The paper itself

Abstract

Advances in cellular reprogramming and stem cell differentiation now enable ex vivo studies of human neuronal differentiation. However, it remains challenging to elucidate the underlying regulatory programs because differentiation protocols are laborious and often result in low neuron yields. Here, we overexpressed two Neurogenin transcription factors in human-induced pluripotent stem cells and obtained neurons with bipolar morphology in 4 days, at greater than 90% purity. The high purity enabled mRNA and microRNA expression profiling during neurogenesis, thus revealing the genetic programs involved in the rapid transition from stem cell to neuron. The resulting cells exhibited transcriptional, morphological and functional signatures of differentiated neurons, with greatest transcriptional similarity to prenatal human brain samples. Our analysis revealed a network of key transcription factors and microRNAs that promoted loss of pluripotency and rapid neurogenesis via progenitor states. Perturbations of key transcription factors affected homogeneity and phenotypic properties of the resulting neurons, suggesting that a systems-level view of the molecular biology of differentiation may guide subsequent manipulation of human stem cells to rapidly obtain diverse neuronal types.

Indexed as

NeurogenesisTranscriptional ActivationBasic Helix-Loop-Helix ProteinsBrainCell DifferentiationCellular ReprogrammingGene Expression ProfilingGene Expression RegulationHumansInduced Pluripotent Stem CellsNerve Tissue ProteinsBasic Helix-Loop-Helix ProteinsNerve Tissue ProteinsNEUROG1 protein, humanNEUROG2 protein, humangene regulatory networksmicroRNAsneurogenesisstem cell differentiationtranscriptomics

Identifiers

PMID25403753
PMCPMC4299601
OpenAlexW2170098894

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.