ArticlePlant molecular biology2015
Comparative assessments of CRISPR-Cas nucleases' cleavage efficiency in planta.
Article in Plant molecular biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
33 citing papers in PubMed, 81 citations in OpenAlex.
- CRISPR-based gene editing in plants: Focus on reagents and their delivery tools.BioImpacts : BI · 2025Review
- New improvements in grapevine genome editing: high efficiency biallelic homozygous knock-out from regenerated plantlets by using an optimized zCas9i.Plant methods · 2024Article
- Revolutionizing Agriculture: Harnessing CRISPR/Cas9 for Crop Enhancement.Indian journal of microbiology · 2024Review
- Crop genome editing through tissue-culture-independent transformation methods.Frontiers in genome editing · 2024Review
- Porcine reproductive and respiratory syndrome virus upregulates SMPDL3B to promote viral replication by modulating lipid metabolism.iScience · 2023Article
- CRISPR-Based Genome Editing: Advancements and Opportunities for Rice Improvement.International journal of molecular sciences · 2022Review
- Spatiotemporal Regulation of CRISPR/Cas9 Enables Efficient, Precise, and Heritable Edits in Plant Genomes.Frontiers in genome editing · 2022Review
- Efficient genome editing and gene knockout inPlant biotechnology (Tokyo, Japan) · 2021Article
- Revisiting CRISPR/Cas-mediated crop improvement: Special focus on nutrition.Journal of biosciences · 2020Article
- The viral F-box protein P0 induces an ER-derived autophagy degradation pathway for the clearance of membrane-bound AGO1.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Target DNA mutagenesis-based fluorescence assessment of off-target activity of the CRISPR-Cas9 system.RSC advances · 2019Article
- A screening method to identify efficient sgRNAs in Arabidopsis, used in conjunction with cell-specific lignin reduction.Biotechnology for biofuels · 2019Article
- CRISPR/Cas9-mediated multiple gene editing inHorticulture research · 2019Article
- CRISPR-Cas9 ribonucleoprotein-mediated co-editing and counterselection in the rice blast fungus.Scientific reports · 2018Article
- A Dual sgRNA Approach for Functional Genomics inG3 (Bethesda, Md.) · 2018Article
- Efficient CRISPR/Cas9-based genome editing in carrot cells.Plant cell reports · 2018Article
- Optimization of CRISPR/Cas9 genome editing in cotton by improved sgRNA expression.Plant methods · 2018Article
- Efficient CRISPR/Cas9-based genome editing and its application to conditional genetic analysis in Marchantia polymorpha.PloS one · 2018Article
- Targeted recombination between homologous chromosomes for precise breeding in tomato.Nature communications · 2017Article
- Application of CRISPR/Cas9 in plant biology.Acta pharmaceutica Sinica. B · 2017Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Custom-designed nucleases can enable precise plant genome editing by catalyzing DNA-breakage at specific targets to stimulate targeted mutagenesis or gene replacement. The CRISPR-Cas system, with its target-specifying RNA molecule to direct the Cas9 nuclease, is a recent addition to existing nucleases that bind and cleave the target through linked protein domains (e.g. TALENs and zinc-finger nucleases). We have conducted a comparative study of these different types of custom-designed nucleases and we have assessed various components of the CRISPR-Cas system. For this purpose, we have adapted our previously reported assay for cleavage-dependent luciferase gene correction in Nicotiana benthamiana leaves (Johnson et al. in Plant Mol Biol 82(3):207-221, 2013). We found that cleavage by CRISPR-Cas was more efficient than cleavage of the same target by TALENs. We also compared the cleavage efficiency of the Streptococcus pyogenes Cas9 protein based on expression using three different Cas9 gene variants. We found significant differences in cleavage efficiency between these variants, with human and Arabidopsis thaliana codon-optimized genes having the highest cleavage efficiencies. We compared the activity of 12 de novo-designed single synthetic guide RNA (sgRNA) constructs, and found their cleavage efficiency varied drastically when using the same Cas9 nuclease. Finally, we show that, for one of the targets tested with our assay, we could induce a germinally-transmitted deletion in a repeat array in A. thaliana. This work emphasizes the efficiency of the CRISPR-Cas system in plants. It also shows that further work is needed to be able to predict the optimal design of sgRNAs or Cas9 variants.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.