Evidence map›Paper›PMID 25403566›Full record

ArticleJournal of dental research2015

NFIB regulates embryonic development of submandibular glands.

R E Mellas, H Kim, J Osinski, S Sadibasic, R M Gronostajski, M Cho, O J Baker

Open access · greenAbstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of dental research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed, 1 pooled it
0.7field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 1 synthesis or guideline pooled it, 24 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Review
  12. Review
  13. Regulatory Mechanisms Driving Salivary Gland Organogenesis.Current topics in developmental biology · 2015
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

R E MellasSchool of Dentistry University of Utah, Salt Lake City, UT, USA.
H KimDepartment of Restorative Dentistry, School of Dental Medicine, University at Buffalo, The State University of New York, Buffalo, NY, USA.
J OsinskiDepartment of Biochemistry, Developmental Genomics Group, Center of Excellence in Bioinformatics and Life Science, University at Buffalo, The State University of New York, Buffalo, NY, USA.
S SadibasicDepartment of Restorative Dentistry, School of Dental Medicine, University at Buffalo, The State University of New York, Buffalo, NY, USA.
R M GronostajskiDepartment of Biochemistry, Developmental Genomics Group, Center of Excellence in Bioinformatics and Life Science, University at Buffalo, The State University of New York, Buffalo, NY, USA.
M ChoDepartment of Oral Biology, School of Dental Medicine, University at Buffalo, The State University of New York, Buffalo, NY, USA.
O J BakerSchool of Dentistry University of Utah, Salt Lake City, UT, USA olga.baker@hsc.utah.edu.
University at Buffalo, State University of New York · USUniversity of Utah · US

Funding

THE USE OF FIBRIN HYDROGELS TO PROMOTE SALIVARY GLAND REGENERATIONR01DE022971 · NIDCR · UNIVERSITY OF UTAH · PI ANDREADIS, STELIOS T., BAKER, OLGA JULIANA · 2012 to 2025
$7.2M
Resolution of Cytokine-Mediated Salivary Gland InflammationR01DE021697 · NIDCR · UNIVERSITY OF UTAH · PI BAKER, OLGA JULIANA · 2012 to 2016
$2.3M
Roles of NFI Genes in Mouse Lung DevelopmentR01HL080624 · NHLBI · STATE UNIVERSITY OF NEW YORK AT BUFFALO · PI GRONOSTAJSKI, RICHARD M · 2007 to 2011
$2.0M
NHLBI NIH HHS HL080624NHLBI NIH HHS R01 HL080624NIDCR NIH HHS R01 DE021697NIDCR NIH HHS R01DE021697NIDCR NIH HHS R01 DE022971NIDCR NIH HHS R01DE022971
6 · The paper itself

Abstract

NFIB (nuclear factor I B) is a NFI transcription factor family member, which is essential for the development of a variety of organ systems. Salivary gland development occurs through several stages, including prebud, bud, pseudoglandular, canalicular, and terminal. Although many studies have been done to understand mouse submandibular gland (SMG) branching morphogenesis, little is known about SMG cell differentiation during the terminal stages. The goal of this study was to determine the role of NFIB during SMG development. We analyzed SMGs from wild-type and Nfib-deficient mice (Nfib (-/-)). At embryonic (E) day 18.5, SMGs from wild-type mice showed duct branching morphogenesis and differentiation of tubule ductal cells into tubule secretory cells. In contrast, SMGs from Nfib (-/-) mice at E18.5 failed to differentiate into tubule secretory cells while branching morphogenesis was unaffected. SMGs from wild-type mice at E16.5 displayed well-organized cuboidal inner terminal tubule cells. However, SMGs from Nfib (-/-) at E16.5 displayed disorganized inner terminal tubule cells. SMGs from wild-type mice at E18.5 became fully differentiated, as indicated by a high degree of apicobasal polarization (i.e., presence of apical ZO-1 and basolateral E-cadherin) and columnar shape. Furthermore, SMGs from wild-type mice at E18.5 expressed the protein SMGC, a marker for tubule secretory cells. However, SMGs from Nfib (-/-) mice at E18.5 showed apicobasal polarity, but they were disorganized and lost the ability to secrete SMGC. These findings indicate that the transcription factor NFIB is not required for branching morphogenesis but plays a key role in tubule cell differentiation during mouse SMG development.

Indexed as

AnimalsAquaporin 5BiomarkersCadherinsCell DifferentiationCell PolarityCell ShapeEmbryonic DevelopmentImmunohistochemistryMiceMice, Inbred C57BLMice, KnockoutMicroscopy, ConfocalMorphogenesisMucinsNFI Transcription FactorsAqp5 protein, mouseAquaporin 5BiomarkersCadherinsMucinsNfib protein, mouseNFI Transcription FactorsSmgc protein, mouseTjp1 protein, mouseZonula Occludens-1 Proteincellsdifferentiationepitheliummorphogenesissalivary glandstranscription factors

Identifiers

PMID25403566
PMCPMC4300299
OpenAlexW2062014243

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.