ArticleBMC pulmonary medicine2014
Analysis of the bacterial community in chronic obstructive pulmonary disease sputum samples by denaturing gradient gel electrophoresis and real-time PCR.
Article in BMC pulmonary medicine, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Potential Association Between Changes in Microbiota Level and Lung Diseases: A Meta-Analysis.Frontiers in medicine · 2021Pooled it
- Gut-Lung Microbiota Characterization in Patients with Non-Small Cell Lung Carcinoma and COVID-19 Coinfection.Archives of Iranian medicine · 2024Article
- The impact of Sangju Qingjie Decoction on the pulmonary microbiota in the prevention and treatment of chronic obstructive pulmonary disease.Frontiers in cellular and infection microbiology · 2024Article
- Lung and Gut Microbiome in COPD.Journal of personalized medicine · 2023Review
- Metabolomics of Respiratory Diseases.Handbook of experimental pharmacology · 2023Review
- Advantages and challenges of metagenomic sequencing for the diagnosis of pulmonary infectious diseases.The clinical respiratory journal · 2022Review
- Association of SARS-CoV-2 and Polypharmacy with Gut-Lung Axis: From Pathogenesis to Treatment.ACS omega · 2022Review
- Periodontal Status and Microbiologic Pathogens in Patients with Chronic Obstructive Pulmonary Disease and Periodontitis: A Case-Control Study.International journal of chronic obstructive pulmonary disease · 2020Article
- Role of Lung Microbiome in Innate Immune Response Associated With Chronic Lung Diseases.Frontiers in medicine · 2020Review
- Article
- How human microbiome talks to health and disease.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2018Review
- Brain-derived neurotrophic factor preserves intestinal mucosal barrier function and alters gut microbiota in mice.The Kaohsiung journal of medical sciences · 2018Article
- Microbiological airway colonization in COPD patients with severe emphysema undergoing endoscopic lung volume reduction.International journal of chronic obstructive pulmonary disease · 2018Article
- Role of microbiota on lung homeostasis and diseases.Science China. Life sciences · 2017Review
- Desired Turbulence? Gut-Lung Axis, Immunity, and Lung Cancer.Journal of oncology · 2017Review
- Potential of Health and Demographic Surveillance System in Asthma and Chronic Obstructive Pulmonary Disease Microbiome Research.Frontiers in public health · 2017Article
- Understanding persistent bacterial lung infections: clinical implications informed by the biology of the microbiota and biofilms.Clinical pulmonary medicine · 2016Article
- The Microbiome and the Respiratory Tract.Annual review of physiology · 2016Review
- Bacterial-viral load and the immune response in stable and exacerbated COPD: significance and therapeutic prospects.International journal of chronic obstructive pulmonary disease · 2016Review
- Quantitative analysis of pathogens in the lower respiratory tract of patients with chronic obstructive pulmonary disease.BMC pulmonary medicine · 2015Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe Global Initiative defines COPD for chronic obstructive lung disease as an entirely preventable and treatable disease characterized by sputum production, bacterial colonisation, neutrophilic bronchial airway inflammation and poor health status. The World Health Organization (WHO) estimates that COPD will become the fourth-most common cause of death worldwide, just behind ischemic heart disease, cerebrovascular disease and HIV/AIDS, by 2030. The aim of this study was to determine the main structure feature of sputum potentially pathogenic microorganisms in subjects with COPD during the clinical stable state.
methodsWe employed a molecular genetics-based investigation of the bacteria community, including DNA isolation, PCR amplification and DGGE profiling. PCR-denaturing gradient gel electrophoresis (DGGE) with universal primers targeting the V3 region of the 16S rRNA gene was employed to characterize the overall COPD patient sputum microbiota composition, and some excised gel bands were cloned for sequencing. Real-time PCR was further utilized to quantitatively analyze the subpopulation of microbiota using group-specific primers targeting Streptococcus pneumoniae, Klebsiella pneumoniae, Pseudomonas aeruginosa.
resultsThe DGGE profiles of two groups displayed significant differences between COPD and healthy groups (P < 0.05). Real-time PCR revealed significant increases of Streptococcus pneumoniae, Klebsiella pneumoniae and Pseudomonas aeruginosa (P < 0.05) in the COPD group compared with the healthy group.
conclusionThis study revealed strong relationship between alterations of sputum microbiota and COPD. By determining the content of several types of bacteria, we can provide evidence to aid in the diagnosis and treatment of COPD.
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