ArticleNature structural & molecular biology2014
Structure of AMP-PNP-bound BtuCD and mechanism of ATP-powered vitamin B12 transport by BtuCD-F.
Article in Nature structural & molecular biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed.
- Cobamide Scavenging and Import via a Multiprotein Extracellular Relay in Bacteroides thetaiotaomicron.Chembiochem : a European journal of chemical biology · 2026Review
- Evolutionarily divergent DUF4465 domains have a common vitamin BFEBS open bio · 2026Article
- Drop Dilution Enables the Use of PEG-Derived Detergents for Membrane Protein Purification.ACS omega · 2025Article
- Corrinoid salvaging and cobamide remodeling in bacteria and archaea.Journal of bacteriology · 2024Review
- Characterization of membrane protein interactions by peptidisc-mediated mass photometry.iScience · 2024Article
- Mechanistic insights of ABC importer HutCD involved in heme internalization by Vibrio cholerae.Scientific reports · 2022Article
- Cryo-EM reveals unique structural features of the FhuCDB Escherichia coli ferrichrome importer.Communications biology · 2021Article
- The structural basis of bacterial manganese import.Science advances · 2021Article
- Extracellular haem utilization by the opportunistic pathogen Pseudomonas aeruginosa and its role in virulence and pathogenesis.Advances in microbial physiology · 2021Article
- Structures of ABC transporters: handle with care.FEBS letters · 2020Review
- Article
- Structural basis of substrate recognition by a polypeptide processing and secretion transporter.eLife · 2020Article
- The Different Effects of Substrates and Nucleotides on the Complex Formation of ABC Transporters.Structure (London, England : 1993) · 2019Article
- Noncanonical role for the binding protein in substrate uptake by the MetNI methionine ATP Binding Cassette (ABC) transporter.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Molecular Mechanism of ATP Hydrolysis in an ABC Transporter.ACS central science · 2018Article
- Triple resonance EPR spectroscopy determines the MnJournal of magnetic resonance (San Diego, Calif. : 1997) · 2018Article
- Vitamin BNature chemical biology · 2018Article
- Single-molecule probing of the conformational homogeneity of the ABC transporter BtuCD.Nature chemical biology · 2018Article
- Functional and structural characterization of an ECF-type ABC transporter for vitamin B12.eLife · 2018Article
- Enhanced Solubilization of Class B Radical S-Adenosylmethionine Methylases by Improved Cobalamin Uptake in Escherichia coli.Biochemistry · 2018Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The reaction mechanism of BtuCD-F-catalyzed vitamin B12 transport into Escherichia coli is currently unclear. Here we present the structure of the last missing state in the form of AMP-PNP-bound BtuCD, trapped by a disulfide cross-link. Our structural and biochemical data allow a consistent mechanism to be formulated, thus rationalizing the roles of substrate, ATP and substrate-binding protein.
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