ArticleAMB Express2014
Biodegradation of variable-chain-length n-alkanes in Rhodococcus opacus R7 and the involvement of an alkane hydroxylase system in the metabolism.
Article in AMB Express, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 71 citations in OpenAlex.
- Mechanisms Underlying Efficient n-Eicosane Degradation and Physiological Adaptation in Rhodococcus erythropolis KB1.Journal of basic microbiology · 2026Article
- Genome-to-function integrated exploration of polyethylene biodegradation by Rhodococcus opacus R7.BMC microbiology · 2026Article
- Characterization of C16-C36 alkane degradation and oily sludge bioremediation byApplied and environmental microbiology · 2025Article
- Whole genome of petroleum hydrocarbon degrading Rhodococcus indonesiensis isolated from Nacharam, Hyderabad, India.Scientific reports · 2025Article
- The GntR/VanR transcription regulator AlkR represses AlkB2 monooxygenase expression and regulatesmLife · 2025Article
- Generalization of Classification of AlkB Family Alkane Monooxygenases fromInternational journal of molecular sciences · 2025Article
- Article
- Toward a Circular Bioeconomy: Designing Microbes and Polymers for Biodegradation.ACS synthetic biology · 2024Review
- Autochthonous psychrophilic hydrocarbonoclastic bacteria and its ecological function in contaminated cold environments.Biodegradation · 2024Review
- Exoproteome analysis of Pseudomonas aeruginosa response to high alkane stress.Archives of microbiology · 2024Article
- Insights into polyethylene biodegradative fingerprint ofFrontiers in bioengineering and biotechnology · 2024Article
- Polyethylene Biodegradation by an Artificial Bacterial Consortium: Rhodococcus as a Competitive Plastisphere Species.Microbes and environments · 2024Article
- Insights into the biodegradation of polycaprolactone through genomic analysis of two plastic-degradingFrontiers in microbiology · 2023Article
- Degradation of long-chain n-alkanes by a novel thermal-tolerant Rhodococcus strain.Archives of microbiology · 2022Article
- Significance of both alkB and P450 alkane-degrading systems in Tsukamurella tyrosinosolvens: proteomic evidence.Applied microbiology and biotechnology · 2022Article
- Transcriptomic analysis of Rhodococcus opacus R7 grown on polyethylene by RNA-seq.Scientific reports · 2021Article
- Characterization and Transcriptome Analysis of a Long-ChainInternational journal of environmental research and public health · 2021Article
- Analysis of the biodegradative and adaptive potential of the novel polychlorinated biphenyl degraderMicrobial genomics · 2020Article
- Genome-based analysis for the identification of genes involved in o-xylene degradation in Rhodococcus opacus R7.BMC genomics · 2018Article
- Hydrocarbon degradation potential and plant growth-promoting activity of culturable endophytic bacteria of Lotus corniculatus and Oenothera biennis from a long-term polluted site.Environmental science and pollution research international · 2017Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rhodococcus opacus R7 is a Gram-positive bacterium isolated from a polycyclic aromatic hydrocarbon contaminated soil for its versatile metabolism; indeed the strain is able to grow on naphthalene, o-xylene, and several long- and medium-chain n-alkanes. In this work we determined the degradation of n-alkanes in Rhodococcus opacus R7 in presence of n-dodecane (C12), n-hexadecane (C16), n-eicosane (C20), n-tetracosane (C24) and the metabolic pathway in presence of C12. The consumption rate of C12 was 88%, of C16 was 69%, of C20 was 51% and of C24 it was 78%. The decrement of the degradation rate seems to be correlated to the length of the aliphatic chain of these hydrocarbons. On the basis of the metabolic intermediates determined by the R7 growth on C12, our data indicated that R. opacus R7 metabolizes medium-chain n-alkanes by the primary alcohol formation. This represents a difference in comparison with other Rhodococcus strains, in which a mixture of the two alcohols was observed. By GC-MSD analysis we also identified the monocarboxylic acid, confirming the terminal oxidation. Moreover, the alkB gene cluster from R. opacus R7 was isolated and its involvement in the n-alkane degradation system was investigated by the cloning of this genomic region into a shuttle-vector E. coli-Rhodococcus to evaluate the alkane hydroxylase activity. Our results showed an increased biodegradation of C12 in the recombinant strain R. erythropolis AP (pTipQT1-alkR7) in comparison with the wild type strain R. erythropolis AP. These data supported the involvement of the alkB gene cluster in the n-alkane degradation in the R7 strain.
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