ArticleBiomedical optics express2014
Multimodal nonlinear optical microscopic imaging provides new insights into acetowhitening mechanisms in live mammalian cells without labeling.
Article in Biomedical optics express, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 10 citations in OpenAlex.
- Employing acetowhitening to identify mohs micrographic surgery margins in squamous cell carcinoma in situ.Archives of dermatological research · 2025Article
- Noninvasive morpho-molecular imaging reveals early therapy-induced senescence in human cancer cells.Science advances · 2023Article
- Widely-tunable synchronisation-free picosecond laser source for multimodal CARS, SHG, and two-photon microscopy.Biomedical optics express · 2021Article
- A quantitative framework for the analysis of multimodal optical microscopy images.Quantitative imaging in medicine and surgery · 2017Article
- Spectrally-broad coherent anti-Stokes Raman scattering hyper-microscopy utilizing a Stokes supercontinuum pumped at 800 nm.Biomedical optics express · 2016Article
- Optical diagnosis and characterization of dental caries with polarization-resolved hyperspectral stimulated Raman scattering microscopy.Biomedical optics express · 2016Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
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Abstract
We developed a multimodal nonlinear optical microscopy imaging (e.g., third-harmonic generation (THG) and two-photon excited fluorescence (TPEF)) platform based on a femtosecond laser pumped photonic crystal fiber to investigate the acetowhitening phenomenon induced by acetic acid in live mammalian cells without labeling. After treated by acetic acid with concentrations of higher than 0.2%, THG images show that light scattering is remarkably increased inside the nucleus and cytoplasm in cells. Co-localized TPEF and THG imaging on tryptophan and NADH in cells indicates that the change of scattering property is largely originating from the morphological change of metabolic proteins induced by acetic acids. Further TPEF imaging on NADH and FAD in cells confirms that this change is irreversible when acetic acid concentration is higher than 1.2%. These subcellular-level THG/TPEF imaging results reveal that the acetowhitening phenomenon is highly related with proteins involved in metabolic pathways in the nucleus and cytoplasm in live cells.
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