Evidence map›Paper›PMID 25400721›Full record

ArticleInternational journal of clinical and experimental pathology2014

Preparation of specific anti-Helicobacter pylori yolk antibodies and their antibacterial effects.

Baoning Wang, Jing Yang, Suizhong Cao, Hongren Wang, Xing Pan, Jie Zhu, Yongjun Zhou, Lizhen Gao, Wanyi Li, Mingyuan Li

Open access · greenAbstract read
PubMed Full text at PMC
In one paragraph

Article in International journal of clinical and experimental pathology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
2.8field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 19 citations in OpenAlex.

  1. Trial
  2. Article
  3. Review
  4. Review
  5. IgYs: on her majesty's secret service.Frontiers in immunology · 2023
    Review
  6. Article
  7. Article
  8. Article
  9. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 1 country.

Baoning WangDepartment of Microbiology, West China of Preclinical and Forensic Medicine, Sichuan University Chengdu 610041, China.
Jing YangDepartment of Microbiology, West China of Preclinical and Forensic Medicine, Sichuan University Chengdu 610041, China ; Sichuan Vaccine Technology Co., Ltd. Chengdu 610041, Sichuan, China ; Department of Medical Microbiology, School of Preclinical Medicine, Hubei University of Medicine Shiyan 442000, China.
Suizhong CaoCollege of Veterinary Medicine, Sichuan Agricultural University Ya'an 625014, Sichuan, China.
Hongren WangDepartment of Microbiology, West China of Preclinical and Forensic Medicine, Sichuan University Chengdu 610041, China.
Xing PanDepartment of Microbiology, West China of Preclinical and Forensic Medicine, Sichuan University Chengdu 610041, China ; Sichuan Vaccine Technology Co., Ltd. Chengdu 610041, Sichuan, China.
Jie ZhuSichuan Vaccine Technology Co., Ltd. Chengdu 610041, Sichuan, China.
Yongjun ZhouSichuan Vaccine Technology Co., Ltd. Chengdu 610041, Sichuan, China.
Lizhen GaoSichuan Vaccine Technology Co., Ltd. Chengdu 610041, Sichuan, China.
Wanyi LiDepartment of Microbiology, West China of Preclinical and Forensic Medicine, Sichuan University Chengdu 610041, China.
Mingyuan LiDepartment of Microbiology, West China of Preclinical and Forensic Medicine, Sichuan University Chengdu 610041, China.
Sichuan University · CNScience and Technology Department of Sichuan Province · CNSichuan Agricultural University · CNWest China Medical Center of Sichuan University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTo study immunization procedures and preparation methods of specific IgY antibodies (IgY-Hp, IgY-IB) produced by hens immunized with Helicobacter pylori (Hp) bacterial antigen and recombinant Hp specific antigen IB, detect the inhibition effects on Hp growth and Hp urease activity, and study the effects of oral administration for treating Hp infection.

methodsBy using recombinant cholera toxin subunit B (rCTB) as an adjuvant, hens received intramuscular injection immunization for continuous 7 times at an interval of 14 days. Then, the eggs were collected; IgY was purified.

resultsOn day 49 after hens were immunized, levels of two antibodies all reached 1:12800; after they were purified by Ammonium sulfate precipitation, their purity was over 80%. IgY-Hp could inhibit Hp growth and inhibit Hp urease activity; although in vitro, IgY-IB could not inhibit Hp growth but could inhibit Hp urease activity. The experiments in vivo found that when IgY-Hp or IgY-IB with sucralfate dual oral therapy was used to treat Hp infected mouse model, the cure rate all could reach 83.3%.

conclusionAccording to immunization procedure, high titer specific IgY antibody (1:12800) can be obtained in 49 days and its titer remains stable. Oral administration of the specific IgY antibodies in Hp infected mice can reach a cure rate of 83.3%, and the antibodies are expected to become new drugs and therapeutic methods of targeted therapy against Hp infection.

Indexed as

Adjuvants, ImmunologicAdministration, OralAnimalsAnti-Bacterial AgentsAntibodies, BacterialChickensCholera ToxinDisease Models, AnimalEgg YolkEscherichia coliFemaleGastritisHelicobacter InfectionsHelicobacter pyloriImmunizationImmunoglobulinsAdjuvants, ImmunologicAnti-Bacterial AgentsAntibodies, BacterialCholera ToxinIgYImmunoglobulinsSucralfateUreaseantibacterial effectHelicobacter pylorispecific IgY

Identifiers

PMID25400721
PMCPMC4230139
OpenAlexW2340687303

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.