ArticleFrontiers in microbiology2014
The genetic basis of energy conservation in the sulfate-reducing bacterium Desulfovibrio alaskensis G20.
Article in Frontiers in microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed, 73 citations in OpenAlex.
- Cobalt starvation affects multiple cellular processes inMicrobial genomics · 2026Article
- Proteomic Evidence for Biofilm-Mediated Copper Stress Adaptation in Oleidesulfovibrio alaskensis G20.Environmental microbiology · 2026Article
- On the function of TRAP substrate-binding proteins: the isethionate-specific binding protein IseP.The Biochemical journal · 2024Article
- Living in mangroves: a syntrophic scenario unveiling a resourceful microbiome.BMC microbiology · 2024Article
- Evolutionary history and origins of Dsr-mediated sulfur oxidation.The ISME journal · 2024Article
- Microbiomes ofFrontiers in microbiology · 2024Article
- Physiological potential and evolutionary trajectories of syntrophic sulfate-reducing bacterial partners of anaerobic methanotrophic archaea.PLoS biology · 2023Article
- Cross-feeding in the gut microbiome: Ecology and mechanisms.Cell host & microbe · 2023Review
- Large-scale genetic characterization of the model sulfate-reducing bacterium,Frontiers in microbiology · 2023Article
- Micellar catalysis of the Suzuki Miyaura reaction using biogenic Pd nanoparticles fromGreen chemistry : an international journal and green chemistry resource : GC · 2021Article
- Gene Sets and Mechanisms of Sulfate-Reducing Bacteria Biofilm Formation and Quorum Sensing With Impact on Corrosion.Frontiers in microbiology · 2021Review
- The Effects of Unfermented and Fermented Cow and Sheep Milk on the Gut Microbiota.Frontiers in microbiology · 2019Article
- Article
- Electron carriers in microbial sulfate reduction inferred from experimental and environmental sulfur isotope fractionations.The ISME journal · 2018Article
- Electron carriers in microbial sulfate reduction inferred from experimental and environmental sulfur isotope fractionations.The ISME journal · 2018Article
- Filling gaps in bacterial amino acid biosynthesis pathways with high-throughput genetics.PLoS genetics · 2018Article
- Flavin-Based Electron Bifurcation, Ferredoxin, Flavodoxin, and Anaerobic Respiration With Protons (Ech) or NADFrontiers in microbiology · 2018Review
- Deconstructing the Dissimilatory Sulfate Reduction Pathway: Isotope Fractionation of a Mutant Unable of Growth on Sulfate.Frontiers in microbiology · 2018Article
- Homoacetogenesis in Deep-SeamBio · 2017Article
- Sulfur Cycling and the Intestinal Microbiome.Digestive diseases and sciences · 2017Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sulfate-reducing bacteria play major roles in the global carbon and sulfur cycles, but it remains unclear how reducing sulfate yields energy. To determine the genetic basis of energy conservation, we measured the fitness of thousands of pooled mutants of Desulfovibrio alaskensis G20 during growth in 12 different combinations of electron donors and acceptors. We show that ion pumping by the ferredoxin:NADH oxidoreductase Rnf is required whenever substrate-level phosphorylation is not possible. The uncharacterized complex Hdr/flox-1 (Dde_1207:13) is sometimes important alongside Rnf and may perform an electron bifurcation to generate more reduced ferredoxin from NADH to allow further ion pumping. Similarly, during the oxidation of malate or fumarate, the electron-bifurcating transhydrogenase NfnAB-2 (Dde_1250:1) is important and may generate reduced ferredoxin to allow additional ion pumping by Rnf. During formate oxidation, the periplasmic [NiFeSe] hydrogenase HysAB is required, which suggests that hydrogen forms in the periplasm, diffuses to the cytoplasm, and is used to reduce ferredoxin, thus providing a substrate for Rnf. During hydrogen utilization, the transmembrane electron transport complex Tmc is important and may move electrons from the periplasm into the cytoplasmic sulfite reduction pathway. Finally, mutants of many other putative electron carriers have no clear phenotype, which suggests that they are not important under our growth conditions, although we cannot rule out genetic redundancy.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.