ArticleJournal of chemical theory and computation2014
Rapid Computational Prediction of Thermostabilizing Mutations for G Protein-Coupled Receptors.
Article in Journal of chemical theory and computation, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 32 citations in OpenAlex.
- One-shot design elevates functional expression levels of a voltage-gated potassium channel.Protein science : a publication of the Protein Society · 2024Article
- Sequence coevolution and structure stabilization modulate olfactory receptor expression.Biophysical journal · 2022Article
- Development of enhanced conformational sampling methods to probe the activation landscape of GPCRs.Advances in protein chemistry and structural biology · 2022Review
- An online GPCR structure analysis platform.Nature structural & molecular biology · 2021Article
- A novel high-throughput screen for identifying lipids that stabilise membrane proteins in detergent based solution.PloS one · 2021Article
- IMPROvER: the Integral Membrane Protein Stability Selector.Scientific reports · 2020Article
- Theoretical identification of thermostabilizing amino acid mutations for G-protein-coupled receptors.Biophysical reviews · 2020Review
- Machine Learning for Prioritization of Thermostabilizing Mutations for G-Protein Coupled Receptors.Biophysical journal · 2019Article
- Prediction of Conformation Specific Thermostabilizing Mutations for Class A G Protein-Coupled Receptors.Journal of chemical information and modeling · 2019Article
- Engineering Salt Bridge Networks between Transmembrane Helices Confers Thermostability in G-Protein-Coupled Receptors.Journal of chemical theory and computation · 2018Article
- Computational design of thermostabilizing point mutations for G protein-coupled receptors.eLife · 2018Article
- Investigation of allosteric modulation mechanism of metabotropic glutamate receptor 1 by molecular dynamics simulations, free energy and weak interaction analysis.Scientific reports · 2016Article
- How Can Mutations Thermostabilize G-Protein-Coupled Receptors?Trends in pharmacological sciences · 2016Review
- Identifying Thermostabilizing Mutations in Membrane Proteins by Bioinformatics.Biophysical journal · 2015Article
- Rapid Bioinformatic Identification of Thermostabilizing Mutations.Biophysical journal · 2015Article
- Large-scale production and protein engineering of G protein-coupled receptors for structural studies.Frontiers in pharmacology · 2015Review
- Stabilization of G protein-coupled receptors by point mutations.Frontiers in pharmacology · 2015Review
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 2 countries.
Funding
Abstract
G protein-coupled receptors (GPCRs) are highly dynamic and often denature when extracted in detergents. Deriving thermostable mutants has been a successful strategy to stabilize GPCRs in detergents, but this process is experimentally tedious. We have developed a computational method to predict the position of the thermostabilizing mutations for a given GPCR sequence. We have validated the method against experimentally measured thermostability data for single mutants of the β
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.