Evidence map›Paper›PMID 25399436›Full record

ArticleAmerican journal of physiology. Lung cellular and molecular physiology2015

Hyperoxia-induced changes in estradiol metabolism in postnatal airway smooth muscle.

Yvette N Martin, Logan Manlove, Jie Dong, William A Carey, Michael A Thompson, Christina M Pabelick, Hitesh C Pandya, Richard J Martin, Dennis A Wigle, Y S Prakash

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in American journal of physiology. Lung cellular and molecular physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
1.9field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 18 citations in OpenAlex.

  1. Review
  2. Sex-Steroid Signaling in Lung Diseases and Inflammation.Advances in experimental medicine and biology · 2021
    Article
  3. Article
  4. It's about time: clocks in the developing lung.The Journal of clinical investigation · 2020
    Review
  5. Article
  6. Article
  7. Review
  8. Sex-specific differences in neonatal hyperoxic lung injury.American journal of physiology. Lung cellular and molecular physiology · 2016
    Article
  9. Coming to terms with tissue engineering and regenerative medicine in the lung.American journal of physiology. Lung cellular and molecular physiology · 2015
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 2 countries.

Yvette N MartinDepartment of Anesthesiology, Mayo Clinic, Rochester, Minnesota;
Logan ManloveDepartment of Anesthesiology, Mayo Clinic, Rochester, Minnesota;
Jie DongDepartment of Surgery, Mayo Clinic, Rochester, Minnesota;
William A CareyDivision of Neonatal Medicine Mayo Clinic, Rochester, Minnesota.
Michael A ThompsonDepartment of Anesthesiology, Mayo Clinic, Rochester, Minnesota;
Christina M PabelickDepartment of Anesthesiology, Mayo Clinic, Rochester, Minnesota; Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, Minnesota;
Hitesh C PandyaDepartment of Pediatrics, University of Leicester, Leicester, United Kingdom;
Richard J MartinDepartment of Pediatrics, Division of Neonatology, Rainbow Babies Children's Hospital, Case Western Reserve University, Cleveland, Ohio; and.
Dennis A WigleDepartment of Surgery, Mayo Clinic, Rochester, Minnesota;
Y S PrakashDepartment of Anesthesiology, Mayo Clinic, Rochester, Minnesota; Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, Minnesota; Prakash.ys@mayo.edu.
Mayo Clinic · USCase Western Reserve University · USUniversity of Leicester · GB

Funding

Mayo Clinic Center for Translational Science ActivitiesUL1TR000135 · NCATS · MAYO CLINIC ROCHESTER · PI KHOSLA, SUNDEEP · 2012 to 2015
$41.2M
NEURAL REGULATION OF AIRWAYS DURING POSTNATAL MATURATIONR01HL056470 · NHLBI · MAYO CLINIC ROCHESTER · PI PRAKASH, Y. S. · 1996 to 2025
$10.5M
Neurotrophins in the LungR01HL088029 · NHLBI · MAYO CLINIC ROCHESTER · PI PRAKASH, Y. S. · 2009 to 2022
$5.9M
NCATS NIH HHS UL1 TR000135NHLBI NIH HHS R01 HL056470NHLBI NIH HHS R01 HL088029
6 · The paper itself

Abstract

Supplemental oxygen, used to treat hypoxia in preterm and term neonates, increases the risk of neonatal lung diseases, such as bronchopulmonary dysplasia (BPD) and asthma. There is a known sex predilection for BPD, but the underlying mechanisms are not clear. We tested the hypothesis that altered, local estradiol following hyperoxia contributes to pathophysiological changes observed in immature lung. In human fetal airway smooth muscle (fASM) cells exposed to normoxia or hyperoxia, we measured the expression of proteins involved in estrogen metabolism and cell proliferation responses to estradiol. In fASM cells, CYP1a1 expression was increased by hyperoxia, whereas hyperoxia-induced enhancement of cell proliferation was blunted by estradiol. Pharmacological studies indicated that these effects were attributable to upregulation of CYP1a1 and subsequent increased metabolism of estradiol to a downstream intermediate 2-methoxyestradiol. Microarray analysis of mouse lung exposed to 14 days of hyperoxia showed the most significant alteration in CYP1a1 expression, with minimal changes in expression of five other genes related to estrogen receptors, synthesis, and metabolism. Our novel results on estradiol metabolism in fetal and early postnatal lung in the context of hyperoxia indicate CYP1a1 as a potential mechanism for the protective effect of estradiol in hyperoxia-exposed immature lung, which may help explain the sex difference in neonatal lung diseases.

Indexed as

2-MethoxyestradiolAnimalsApoptosisAromataseAsthmaBronchopulmonary DysplasiaCatechol O-MethyltransferaseCell HypoxiaCell ProliferationCells, CulturedCytochrome P-450 CYP1A1Cytochrome P-450 CYP1B1EstradiolEstrogen Receptor alphaEstrogen Receptor betaHumans2-MethoxyestradiolAromataseCatechol O-MethyltransferaseCOMT protein, humanCYP19A1 protein, humanCYP1A1 protein, humanCYP1B1 protein, humanCytochrome P-450 CYP1A1Cytochrome P-450 CYP1B1EstradiolEstrogen Receptor alphaEstrogen Receptor betaOxygenReactive Oxygen SpeciesReceptors, EstrogenRNA, Messenger2-methoxyestradiolairway smooth musclebronchopulmonary dysplasiaCYP1a1estradiolhyperoxianeonate

Identifiers

PMID25399436
PMCPMC4338940
OpenAlexW2140548795

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.