ArticleAmerican journal of physiology. Lung cellular and molecular physiology2015
Transcriptional mechanisms and protein kinase signaling mediate organic dust induction of IL-8 expression in lung epithelial and THP-1 cells.
Article in American journal of physiology. Lung cellular and molecular physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Aryl hydrocarbon receptor (AhR) regulates organic dust and organic dust-associated bacterial extracellular vesicles-induced airway inflammation via ROS-dependent NFκB and STAT3 activation.Respiratory research · 2026Article
- Redox-sensitive GPCR signaling drives Gq-dependent CaAmerican journal of physiology. Cell physiology · 2026Article
- Intracellular C3 regulates the immune response to infection via NF-κB signaling.Cellular and molecular life sciences : CMLS · 2025Article
- Endoplasmic Reticulum Stress and Unfolded Protein Response Sensor ERN1 Regulates Organic Dust Induction of Lung Inflammation.FASEB bioAdvances · 2025Article
- Purification and characterization of a serine protease from organic dust and elucidation of its inductive effects on lung inflammatory mediators.American journal of physiology. Lung cellular and molecular physiology · 2023Article
- Bacterial extracellular vesicles isolated from organic dust induce neutrophilic inflammation in the lung.American journal of physiology. Lung cellular and molecular physiology · 2020Article
- Organic dust induces inflammatory gene expression in lung epithelial cells via ROS-dependent STAT-3 activation.American journal of physiology. Lung cellular and molecular physiology · 2019Article
- Organic dust inhibits surfactant protein expression by reducing thyroid transcription factor-1 levels in human lung epithelial cells.Innate immunity · 2019Article
- Kinome analyses of inflammatory responses to swine barn dust extract in human bronchial epithelial and monocyte cell lines.Innate immunity · 2018Article
- High throughput genomic sequencing of bioaerosols in broiler chicken production facilities.Microbial biotechnology · 2016Article
- Proteases and oxidant stress control organic dust induction of inflammatory gene expression in lung epithelial cells.Respiratory research · 2016Article
- Gene expression profiling of the effects of organic dust in lung epithelial and THP-1 cells reveals inductive effects on inflammatory and immune response genes.Physiological genomics · 2016Article
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Authors and funding
7 authors.
Funding
Abstract
Exposure to the agricultural work environment is a risk factor for the development of respiratory symptoms and chronic lung diseases. Inflammation is an important contributor to the pathogenesis of tissue injury and disease. Cellular and molecular mechanisms mediating lung inflammatory responses to agricultural dust are not yet fully understood. We studied the effects of poultry dust extract on molecular regulation of interleukin-8 (IL-8), a proinflammatory cytokine, in A549 and Beas2B lung epithelial and THP-1 monocytic cells. Our findings indicate that poultry dust extract potently induces IL-8 levels by increasing IL-8 gene transcription without altering IL-8 mRNA stability. Increase in IL-8 promoter activity was due to enhanced binding of activator protein 1 and NF-κB. IL-8 induction was associated with protein kinase C (PKC) and mitogen-activated protein kinase (MAPK) activation and inhibited by PKC and MAPK inhibitors. IL-8 increase was not inhibited by polymyxin B or l-nitroarginine methyl ester, indicating lack of involvement of lipopolysaccharide and nitric oxide in the induction. Lung epithelial and THP-1 cells share common mechanisms for induction of IL-8 levels. Our findings identify key roles for transcriptional mechanisms and protein kinase signaling pathways for IL-8 induction and provide insights into the mechanisms regulating lung inflammatory responses to organic dust exposure.
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