ArticlePloS one2014
Direct detection and differentiation of pathogenic Leptospira species using a multi-gene targeted real time PCR approach.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 56 citations in OpenAlex.
- Integrated diagnostic approach for Leptospira spp. detection in SMEDI associated reproductive disease of swine.Veterinary and animal science · 2026Article
- Postflood Leptospirosis Outbreak Response Due toOpen forum infectious diseases · 2026Article
- Diagnostic Performance of Two Commercial qPCR Kits forTropical medicine and infectious disease · 2026Article
- Dynamics of Leptospirosis Transmission Within Urban Norway Rat (Transboundary and emerging diseases · 2025Article
- Diagnosis and Clinical Description of Leptospirosis Cases Identified Through Hospital-Based Active Surveillance in Puerto Rico, 2019-2021.Journal of tropical medicine · 2025Article
- Leptospirosis outbreak in Ecuador in 2023: A pilot study for surveillance from a One Health perspective.One health (Amsterdam, Netherlands) · 2024Article
- Potentially zoonotic pathogens and parasites in opportunistically sourced urban brown rats (Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2024Article
- Detection of Leptospira kirschneri in a short-beaked common dolphin (Delphinus delphis delphis) stranded off the coast of southern California, USA.BMC veterinary research · 2024Article
- Seroprevalence and renal carriage of pathogenic Leptospira in livestock in Cotonou, Benin.Veterinary medicine and science · 2024Article
- Exudative glomerulonephritis associated with acute leptospirosis in dogs.Veterinary pathology · 2024Article
- Case Report: Locally Acquired Leptospirosis in a Minnesota Boy and His Dog.The American journal of tropical medicine and hygiene · 2024Article
- Genotyping ofFrontiers in microbiology · 2024Article
- Detection and Identification of PathogenicTransboundary and emerging diseases · 2024Article
- Comprehensive diagnostic testing identifies diverse aetiologies of acute febrile illness among hospitalised children and adults in Sri Lanka: a prospective cohort study.BMJ public health · 2023Article
- Examination on the Occurrence of Coinfections in Diagnostic Transmittals in Cases of Stillbirth, Mummification, Embryonic Death, and Infertility (SMEDI) Syndrome in Germany.Microorganisms · 2023Article
- DNA Capture and Enrichment: A Culture-Independent Approach for Characterizing the Genomic Diversity of PathogenicMicroorganisms · 2023Article
- Bacterial and Viral Pathogens with One Health Relevance in Invasive Raccoons (Pathogens (Basel, Switzerland) · 2023Article
- Molecular and Serological Identification of PathogenicTransboundary and emerging diseases · 2023Article
- First Molecular Detection of PathogensTropical medicine and infectious disease · 2022Article
- Comparison of TwoVeterinary sciences · 2022Article
Corrections and comments
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Authors and funding
9 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Leptospirosis is a growing public and veterinary health concern caused by pathogenic species of Leptospira. Rapid and reliable laboratory tests for the direct detection of leptospiral infections in animals are in high demand not only to improve diagnosis but also for understanding the epidemiology of the disease. In this work we describe a novel and simple TaqMan-based multi-gene targeted real-time PCR approach able to detect and differentiate Leptospira interrogans, L. kirschneri, L. borgpeteresenii and L. noguchii, which constitute the veterinary most relevant pathogenic species of Leptospira. The method uses sets of species-specific probes, and respective flanking primers, designed from ompL1 and secY gene sequences. To monitor the presence of inhibitors, a duplex amplification assay targeting both the mammal β-actin and the leptospiral lipL32 genes was implemented. The analytical sensitivity of all primer and probe sets was estimated to be <10 genome equivalents (GE) in the reaction mixture. Application of the amplification reactions on genomic DNA from a variety of pathogenic and non-pathogenic Leptospira strains and other non-related bacteria revealed a 100% analytical specificity. Additionally, pathogenic leptospires were successfully detected in five out of 29 tissue samples from animals (Mus spp., Rattus spp., Dolichotis patagonum and Sus domesticus). Two samples were infected with L. borgpetersenii, two with L. interrogans and one with L. kirschneri. The possibility to detect and identify these pathogenic agents to the species level in domestic and wildlife animals reinforces the diagnostic information and will enhance our understanding of the epidemiology of leptopirosis.
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