ArticleBioconjugate chemistry2014
Small quantum dots conjugated to nanobodies as immunofluorescence probes for nanometric microscopy.
Article in Bioconjugate chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Quantum Dot-Fab' Conjugates as Compact Immunolabels for Microtubule Imaging and Cell Classification.ACS nano · 2024Article
- A framework to validate fluorescently labeled DNA-binding proteins for single-molecule experiments.Cell reports methods · 2023Article
- Quantum Dot-Antibody Conjugates for Immunofluorescence Studies of Biomolecules and Subcellular Structures.Journal of fluorescence · 2022Review
- Recombinant Antibodies in Basic Neuroscience Research.Current protocols in neuroscience · 2020Review
- Phenotypic analysis of extracellular vesicles: a review on the applications of fluorescence.Journal of extracellular vesicles · 2020Review
- Nanobody-Based Probes for Subcellular Protein Identification and Visualization.Frontiers in cellular neuroscience · 2020Review
- Generation and Application of Fluorescent Anti-Human β2-Microglobulin VHHs via Amino Modification.Molecules (Basel, Switzerland) · 2019Article
- Preparation and characterization of a single-domain antibody specific for the porcine epidemic diarrhea virus spike protein.AMB Express · 2019Article
- A fluorometric clenbuterol immunoassay using sulfur and nitrogen doped carbon quantum dots.Mikrochimica acta · 2019Article
- Quantum dot conjugated nanobodies for multiplex imaging of protein dynamics at synapses.Nanoscale · 2018Article
- Single- and two-photon imaging of human micrometastases and disseminated tumour cells with conjugates of nanobodies and quantum dots.Scientific reports · 2018Article
- Article
- Under the Microscope: Single-Domain Antibodies for Live-Cell Imaging and Super-Resolution Microscopy.Frontiers in immunology · 2017Review
- Article
- Control of Transmembrane Protein Diffusion within the Postsynaptic Density Assessed by Simultaneous Single-Molecule Tracking and Localization Microscopy.Frontiers in synaptic neuroscience · 2016Article
- Super resolution microscopy is poised to reveal new insights into the formation and maturation of dendritic spines.F1000Research · 2016Review
- Mechanical transduction by ion channels: A cautionary tale.World journal of neurology · 2015Article
Corrections and comments
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Authors and funding
12 authors.
Funding
Abstract
Immunofluorescence, a powerful technique to detect specific targets using fluorescently labeled antibodies, has been widely used in both scientific research and clinical diagnostics. The probes should be made with small antibodies and high brightness. We conjugated GFP binding protein (GBP) nanobodies, small single-chain antibodies from llamas, with new ∼7 nm quantum dots. These provide simple and versatile immunofluorescence nanoprobes with nanometer accuracy and resolution. Using the new probes we tracked the walking of individual kinesin motors and measured their 8 nm step sizes; we tracked Piezo1 channels, which are eukaryotic mechanosensitive channels; we also tracked AMPA receptors on living neurons. Finally, we used a new super-resolution algorithm based on blinking of (small) quantum dots that allowed ∼2 nm precision.
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Registered trials
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