ArticlePloS one2014
Cell population kinetics of collagen scaffolds in ex vivo oral wound repair.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed, 25 citations in OpenAlex.
- Stability of Root Coverage Outcomes After Soft-Tissue Augmentation With a Collagen Matrix With or Without rhPDGF-BB: A 3-Year Triple-Blinded, Randomised, Placebo-Controlled Trial.Journal of clinical periodontology · 2025Trial
- Tissue Perfusion and Biomarkers Assessment Following Root Coverage Procedures.Journal of periodontal research · 2025Trial
- Comparative evaluation of efficacy of volume stable collagen matrix in interdental papilla reconstruction as compared to connective tissue graft in maxillary esthetic region: a randomized controlled clinical trial.Clinical oral investigations · 2025Trial
- Recombinant human platelet-derived growth factor improves root coverage of a collagen matrix for multiple adjacent gingival recessions: A triple-blinded, randomized, placebo-controlled trial.Journal of clinical periodontology · 2022Trial
- Emerging, personalized, and minimally invasive technologies for periodontal and peri-implant soft and hard tissue regenerative therapy.Journal of periodontology · 2026Review
- Histological evaluation of palatal donor site healing with leukocyte-platelet-rich fibrin versus collagen sponge.Journal of clinical and experimental dentistry · 2025Review
- In Vitro Investigation of Gelatin/Polycaprolactone Nanofibers in Modulating Human Gingival Mesenchymal Stromal Cells.Materials (Basel, Switzerland) · 2023Article
- Altered expression of SARS-CoV-2 entry and processing genes by Porphyromonas gingivalis-derived lipopolysaccharide, inflammatory cytokines and prostaglandin EArchives of oral biology · 2021Article
- Impact of PDGF-BB on cellular distribution and extracellular matrix in the healing rabbit Achilles tendon three weeks post-operation.FEBS open bio · 2020Article
- Integrated Interaction Network of MicroRNA Target Genes in Keloid Scarring.Molecular diagnosis & therapy · 2019Article
- The role of sclerostin and dickkopf-1 in oral tissues - A review from the perspective of the dental disciplines.F1000Research · 2019Review
- Contraction Dynamics of Rod Microtissues of Gingiva-Derived and Periodontal Ligament-Derived Cells.Frontiers in physiology · 2018Article
- Protein nanocoatings on synthetic polymeric nanofibrous membranes designed as carriers for skin cells.International journal of nanomedicine · 2017Article
- Growth factor enriched collagen matrix versus de-epithelialized graft in root coverage procedures - A randomized parallel arm clinical trial.Journal of oral biology and craniofacial researchArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Biodegradable collagen scaffolds are used clinically for oral soft tissue augmentation to support wound healing. This study sought to provide a novel ex vivo model for analyzing healing kinetics and gene expression of primary human gingival fibroblasts (hGF) within collagen scaffolds. Sponge type and gel type scaffolds with and without platelet-derived growth factor-BB (PDGF) were assessed in an hGF containing matrix. Morphology was evaluated with scanning electron microscopy, and hGF metabolic activity using MTT. We quantitated the population kinetics within the scaffolds based on cell density and distance from the scaffold border of DiI-labled hGFs over a two-week observation period. Gene expression was evaluated with gene array and qPCR. The sponge type scaffolds showed a porous morphology. Absolute cell number and distance was higher in sponge type scaffolds when compared to gel type scaffolds, in particular during the first week of observation. PDGF incorporated scaffolds increased cell numbers, distance, and formazan formation in the MTT assay. Gene expression dynamics revealed the induction of key genes associated with the generation of oral tissue. DKK1, CYR61, CTGF, TGFBR1 levels were increased and integrin ITGA2 levels were decreased in the sponge type scaffolds compared to the gel type scaffold. The results suggest that this novel model of oral wound healing provides insights into population kinetics and gene expression dynamics of biodegradable scaffolds.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.