ArticlePloS one2014
Residual antibiotics in decontaminated human cardiovascular tissues intended for transplantation and risk of falsely negative microbiological analyses.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 31 citations in OpenAlex.
- Evaluation of Tissue-C and CorneaMax organ culture media for microbial contamination detection in corneal grafts using the BD BACTEC FX system.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2026Article
- Attenuation of Residual Antibiotics Using Vitamin C in Homograft Heart Valves.Research in heart yield and translational medicine · 2026Article
- Comparing the efficacy of two antibiotic cocktails in decontamination of cardiovascular tissues.Cell and tissue banking · 2025Article
- Microbiological assessment of arterial allografts processed in a tissue bank.Cell and tissue banking · 2021Article
- Killing efficacy of a new hypothermic corneal storage medium against the micro-organisms frequently found in human donor cornea intended for transplantation.BMJ open ophthalmology · 2021Article
- Significant variation in heart valve banking practice.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2019Article
- Validation of microbiological testing in cardiovascular tissue establishments: results of a second international quality-round trial.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2019Article
- Microbial contamination and tissue procurement location: A conventional operating room is not mandatory. An observational study.PloS one · 2019Observational
- Experimental procedures for decontamination and microbiological testing in cardiovascular tissue banks.Experimental biology and medicine (Maywood, N.J.) · 2018Review
- Increased sensitivity of microbiological testing of cornea organ culture medium by additional resin treatment.BMJ open ophthalmology · 2018Article
- Cryopreserved human aortic root allografts arterial wall: Structural changes occurring during thawing.PloS one · 2017Article
- Method for sterility testing of corneal storage and transport media after removal of interfering antimicrobials: prospective validation study in compliance with the European Pharmacopoeia.BMJ open ophthalmology · 2017Article
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Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We investigated the presence of antibiotics in cryopreserved cardiovascular tissues and cryopreservation media, after tissue decontamination with antibiotic cocktails, and the impact of antibiotic residues on standard tissue bank microbiological analyses. Sixteen cardiovascular tissues were decontaminated with bank-prepared cocktails and cryopreserved by two different tissue banks according to their standard operating procedures. Before and after decontamination, samples underwent microbiological analysis by standard tissue bank methods. Cryopreserved samples were tested again with and without the removal of antibiotic residues using a RESEP tube, after thawing. Presence of antibiotics in tissue homogenates and processing liquids was determined by a modified agar diffusion test. All cryopreserved tissue homogenates and cryopreservation media induced important inhibition zones on both Staphylococcus aureus- and Pseudomonas aeruginosa-seeded plates, immediately after thawing and at the end of the sterility test. The RESEP tube treatment markedly reduced or totally eliminated the antimicrobial activity of tested tissues and media. Based on standard tissue bank analysis, 50% of tissues were found positive for bacteria and/or fungi, before decontamination and 2 out of 16 tested samples (13%) still contained microorganisms after decontamination. After thawing, none of the 16 cryopreserved samples resulted positive with direct inoculum method. When the same samples were tested after removal of antibiotic residues, 8 out of 16 (50%) were contaminated. Antibiotic residues present in tissue allografts and processing liquids after decontamination may mask microbial contamination during microbiological analysis performed with standard tissue bank methods, thus resulting in false negatives.
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